Rabbit Recombinant Monoclonal Galanin antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88707.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136086.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77717.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25168.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (AB255700)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may regulate diverse physiologic functions including contraction of smooth muscle of the gastrointestinal and genitourinary tract, growth hormone and insulin release and adrenal secretion.
Alternative names
GAL1, GALN, GLNN, GAL, Galanin peptides
Recommended products
Rabbit Recombinant Monoclonal Galanin antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22496-308
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255700 is the carrier-free version of Anti-Galanin antibody [EPR22496-308] ab254556.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galanin also known as the galanin peptide is a neuropeptide that plays a role in various physiological processes. It has a molecular mass of approximately 3 kDa and is expressed widely throughout the central and peripheral nervous systems. Galanin is extensively distributed in the brain spinal cord and gut indicating its involvement in multiple neural functions.
- Biological function summary
Galanin influences neurotransmitter release and modulates synaptic transmission. It often forms part of a receptor complex with galanin receptors GALR1 GALR2 and GALR3 which mediate its effects. Galanin exerts diverse actions including regulation of mood feeding behavior and modulation of nociception. These broad biological roles highlight its importance in maintaining homeostasis.
- Pathways
Galanin takes part in the regulation of several key signaling pathways such as the MAPK/ERK and the cAMP-PKA pathways. It interacts with other neuropeptides and its activity can modulate the action of neurotransmitters like norepinephrine and serotonin which influence mood and stress response. These pathways reveal its integrative role in neurophysiological regulation and its connection with multiple proteins.
- Associated diseases and disorders
Galanin is linked with conditions such as depression and epilepsy. Changes in galanin expression have been observed in depressive disorders where it may interact with serotonin transporter proteins affecting mood regulation. In epilepsy galanin has a potential role in reducing seizure susceptibility possibly in relation to its interaction with the gamma-aminobutyric acid (GABA) system. Understanding these associations aids in exploring galanin as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88707.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
Immunohistochemical analysis of paraffin-embedded human pituitary gland tissue labeling Galanin with Anti-Galanin antibody [EPR22496-308] ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human pituitary gland (PMID: 10098517) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Galanin antibody [EPR22496-308] ab254556 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galanin antibody [EPR22496-308] ab254556).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136086.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Galanin with Anti-Galanin antibody [EPR22496-308] ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on myenteric nerves of human colon (PMID: 2417157) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Galanin antibody [EPR22496-308] ab254556 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galanin antibody [EPR22496-308] ab254556).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77717.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling Galanin with Anti-Galanin antibody [EPR22496-308] ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse pituitary gland (PMID: 10098517) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Galanin antibody [EPR22496-308] ab254556 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galanin antibody [EPR22496-308] ab254556).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25168.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling Galanin with Anti-Galanin antibody [EPR22496-308] ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat pituitary gland (PMID: 10098517) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Galanin antibody [EPR22496-308] ab254556 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galanin antibody [EPR22496-308] ab254556).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Galanin with Anti-Galanin antibody [EPR22496-308] ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on human liver (PMID: 2448788) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Galanin antibody [EPR22496-308] ab254556 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galanin antibody [EPR22496-308] ab254556).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23019.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Galanin with Anti-Galanin antibody [EPR22496-308] ab254556 at 1/4000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse spleen (PMID: 2448788) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-Galanin antibody [EPR22496-308] ab254556 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galanin antibody [EPR22496-308] ab254556).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700), expandable thumbnail](https://content.abcam.com/products/images/galanin-antibody-epr22496-308-bsa-and-azide-free-ab255700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img23019.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galanin antibody [EPR22496-308] - BSA and Azide free (ab255700)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com