AB155277

Anti-GALE antibody [EPR11089(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GALE antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-galactose 4-epimerase, UDP-GalNAc 4-epimerase, UDP-GlcNAc 4-epimerase, GALE

1 Images

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Western blot - Anti-GALE antibody [EPR11089(B)] (AB155277)

All lanes:

Western blot - Anti-GALE antibody [EPR11089(B)] (ab155277) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HT29 lysate at 10 µg

Lane 3:

HepG2 lysate at 10 µg

Predicted band size: 38 kDa

false

Carrier free

Anti-GALE antibody [EPR11089(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11089(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'GALE' also known as UDP-galactose-4-epimerase is an enzyme that plays a critical role in carbohydrate metabolism. It weighs approximately 38 kDa and is expressed in various tissues across the body. This enzyme is responsible for the interconversion of UDP-galactose and UDP-glucose essential in the Leloir pathway of galactose metabolism. GALE's activity is necessary for maintaining balanced sugar levels and genetic mutations in this protein can lead to significant metabolic issues.

Biological function summary

UDP-galactose-4-epimerase facilitates the conversion of galactose-1-phosphate to UDP-galactose a reaction occurring in the utilization of galactose obtained from diet. This enzyme is not part of a larger protein complex but operates independently to mediate the transformation of sugars. Its activity helps in the synthesis of glycoproteins and glycolipids which are fundamental to various cellular functions including cell signaling and adhesion.

Pathways

GALE is an important component in the galactose metabolism pathway. It ensures the proper function of carbohydrate interconversion processes important for energy production and biomolecule synthesis. GALE interrelates with other enzymes like GALT (galactose-1-phosphate uridyltransferase) and GALK1 (galactokinase 1) which together facilitate the orderly breakdown and use of galactose in cells. Disruption in its function can affect these interconnected pathways leading to metabolic imbalances.

Deficiencies or mutations in GALE lead to type III galactosemia which is characterized by the body's inability to properly manage galactose. This genetic disorder can cause symptoms such as jaundice cataracts and developmental delays. GALE's relationship with GALT another enzyme implicated in classic galactosemia (type I) highlights an interconnected network where disruptions in similar pathways can result in metabolic dysfunctions. Careful monitoring of these enzymes is necessary in managing and understanding the metabolic disorders tied to galactose metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes two distinct but analogous reactions : the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

See full target information GALE

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106670 PubMed37168577

2023

Casein kinase 1α is required to maintain murine hypothalamic pro-opiomelanocortin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyang Lu,Jinglin Zhang,Bingjie Wang,Qiao Gao,Kezhe Ma,Shaona Pei,Juxue Li,Sheng Cui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GALE antibody [EPR11089(B)]

Western blot - Anti-GALE antibody [EPR11089(B)] (AB155277)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise