AB118033

Anti-GALE antibody [OTI1C4]

Name: Anti-GALE antibody [OTI1C4] (ab118033) | Abcam
Brand: Abcam Limited
SKU: AB118033

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(2 Publications)

Mouse Monoclonal GALE antibody. Suitable for IHC-P, WB and reacts with Human, African green monkey, Dog, Rat, Transfected cell lysate samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human GALE.

View Alternative Names

UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-galactose 4-epimerase, UDP-GalNAc 4-epimerase, UDP-GlcNAc 4-epimerase, GALE

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human lymph node tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human bladder carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human prostate carcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human prostate tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human breast adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human liver tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human kidney tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human ovary adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Paraffin-embedded human colon adenocarcinoma tissue stained for GALE using ab118033 at 1/150 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

All lanes:

Western blot - Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

HT-29 (Human colorectal adenocarcinoma cell line) cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (Canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (Human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 38 kDa

false

Supplier Data

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

All lanes:

Western blot - Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1:

Human testis extract at 10 µg

Lane 2:

Human omentum extract at 10 µg

Lane 3:

Human uterus extract at 10 µg

Lane 4:

Human breast extract at 10 µg

Lane 5:

Human brain extract at 10 µg

Lane 6:

Human liver extract at 10 µg

Lane 7:

Human ovary extract at 10 µg

Lane 8:

Human thyroid gland extract at 10 µg

Lane 9:

Human colon extract at 10 µg

Lane 10:

Human spleen extract at 10 µg

Predicted band size: 38 kDa

false

Unknown

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

All lanes:

Western blot - Anti-GALE antibody [OTI1C4] (ab118033) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY GALE cDNA at 5 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1C4

Isotype

IgG1

Carrier free

Reacts with

Rat, Dog, Human, African green monkey

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human GALE.

Q14376

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Product details

The clone number has been updated from 1C4 to OTI1C4, both clone numbers name the same clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'GALE' also known as UDP-galactose-4-epimerase is an enzyme that plays a critical role in carbohydrate metabolism. It weighs approximately 38 kDa and is expressed in various tissues across the body. This enzyme is responsible for the interconversion of UDP-galactose and UDP-glucose essential in the Leloir pathway of galactose metabolism. GALE's activity is necessary for maintaining balanced sugar levels and genetic mutations in this protein can lead to significant metabolic issues.

Biological function summary

UDP-galactose-4-epimerase facilitates the conversion of galactose-1-phosphate to UDP-galactose a reaction occurring in the utilization of galactose obtained from diet. This enzyme is not part of a larger protein complex but operates independently to mediate the transformation of sugars. Its activity helps in the synthesis of glycoproteins and glycolipids which are fundamental to various cellular functions including cell signaling and adhesion.

Pathways

GALE is an important component in the galactose metabolism pathway. It ensures the proper function of carbohydrate interconversion processes important for energy production and biomolecule synthesis. GALE interrelates with other enzymes like GALT (galactose-1-phosphate uridyltransferase) and GALK1 (galactokinase 1) which together facilitate the orderly breakdown and use of galactose in cells. Disruption in its function can affect these interconnected pathways leading to metabolic imbalances.

Deficiencies or mutations in GALE lead to type III galactosemia which is characterized by the body's inability to properly manage galactose. This genetic disorder can cause symptoms such as jaundice cataracts and developmental delays. GALE's relationship with GALT another enzyme implicated in classic galactosemia (type I) highlights an interconnected network where disruptions in similar pathways can result in metabolic dysfunctions. Careful monitoring of these enzymes is necessary in managing and understanding the metabolic disorders tied to galactose metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes two distinct but analogous reactions : the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.

See full target information GALE

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 295:1225-1239 PubMed31819007

2019

Human UDP-galactose 4'-epimerase (GALE) is required for cell-surface glycome structure and function.

Applications

Unspecified application

Species

Unspecified reactive species

Alex Broussard,Alyssa Florwick,Chelsea Desbiens,Nicole Nischan,Corrina Robertson,Ziqiang Guan,Jennifer J Kohler,Lance Wells,Michael Boyce

Cell cycle (Georgetown, Tex.) 11:4174-80 PubMed23070475

2012

Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed F Salem,Diana Whitaker-Menezes,Anthony Howell,Federica Sotgia,Michael P Lisanti

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GALE antibody [OTI1C4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALE antibody [OTI1C4] (AB118033)

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

Western blot - Anti-GALE antibody [OTI1C4] (AB118033)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise