Anti-Galectin 1 antibody

• Flow Cyt

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Flow Cytometry - Anti-Galectin 1 antibody (AB112525)

Overlay histogram showing PC-3 cells stained with ab112525 (Blue).The cells were blocked with 10% normal goat serum. And then incubated with ab112525 at 1µg/1x10^6 cells for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG at 5-10µg/1x10^6 cells was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green) was rabbit IgG (1µg/1x10^6) used under the same conditions. Unlabelled sample (Red) was also used as a control.

• ICC

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Immunocytochemistry - Anti-Galectin 1 antibody (AB112525)

ab112525 staining Galectin 1 in U20S tissue by Immunohistochemistry. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1 : 100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody (AB112525)

Galectin-1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml ab112525 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody (AB112525)

ab112525 staining Galectin 1 in intestinal cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody (AB112525)

ab112525 staining Galectin 1 in gastric cancer tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody (AB112525)

ab112525 (1 μg/mL) staining Galectin 1 in placenta tissue by Immunohistochemistry (Frozen sections)

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody (AB112525)

ab112525 staining Galectin 1 in tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

• WB

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Western blot - Anti-Galectin 1 antibody (AB112525)

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab112525 at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15 kDa. The expected band size for Galectin 1 is at 15 kDa.

All lanes:

Western blot - Anti-Galectin 1 antibody (ab112525) at 0.5 µg/mL

Lane 1:

human Hela whole cell lysates at 30 µg

Lane 2:

human A549 whole cell lysates at 30 µg

Lane 3:

human A375 whole cell lysates at 30 µg

Lane 4:

human MCF-7 whole cell lysates at 30 µg

Lane 5:

rat kidney tissue lysates at 30 µg

Lane 6:

rat PC-12 whole cell lysates at 30 µg

Lane 7:

mouse NIH/3T3 whole cell lysates at 30 µg

Secondary

All lanes:

goat anti-rabbit IgG-HRP at 1/5000 dilution

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