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AB154351

Anti-Galectin 1 antibody

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(2 Publications)

Rabbit Polyclonal Galectin 1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS1 aa 1 to C-terminus.

View Alternative Names

Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody (AB154351)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody (AB154351)

Immunofluorescence analysis of methanol-fixed A431 cells labeling Galectin 1 with ab154351 at 1/200 dilution. Bottom panel shows counterstaining with Hoechst 33342.

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody (AB154351)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody (AB154351)

Immunofluorescence analysis of A549 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Galectin 1 protein at cytoplasm using ab154351 at a 1/500 dilution (Green). Phalloidin, a cytoskeleton marker stained at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody (AB154351)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody (AB154351)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Galectin 1 protein at cytoplasm and nucleus using ab154351 at a 1/200 dilution (Green). Phalloidin, a cytoskeleton marker stained at a 1/200 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunoprecipitation - Anti-Galectin 1 antibody (AB154351)
  • IP

Supplier Data

Immunoprecipitation - Anti-Galectin 1 antibody (AB154351)

Samples were separated by 15% SDS-PAGE. The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody diluted at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Galectin 1 antibody (ab154351) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

Control with 2.5 µg of pre-immune rabbit IgG

Lane 3:

HeLa whole cell lysate (+)

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 15 kDa

false

Western blot - Anti-Galectin 1 antibody (AB154351)
  • WB

Supplier Data

Western blot - Anti-Galectin 1 antibody (AB154351)

Samples were separated by 15% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Galectin 1 antibody (ab154351) at 1/2000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts at 30 µg

Lane 3:

HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 15 kDa

false

Western blot - Anti-Galectin 1 antibody (AB154351)
  • WB

Lab

Western blot - Anti-Galectin 1 antibody (AB154351)

All lanes:

Western blot - Anti-Galectin 1 antibody (ab154351) at 1/500 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

Galectin 1 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 15 kDa

false

Western blot - Anti-Galectin 1 antibody (AB154351)
  • WB

Unknown

Western blot - Anti-Galectin 1 antibody (AB154351)

15% SDS PAGE

All lanes:

Western blot - Anti-Galectin 1 antibody (ab154351) at 1/10000 dilution

All lanes:

NCI-H929 whole cell lysate at 30 µg

Predicted band size: 15 kDa

false

Western blot - Anti-Galectin 1 antibody (AB154351)
  • WB

Unknown

Western blot - Anti-Galectin 1 antibody (AB154351)

15% SDS PAGE

All lanes:

Western blot - Anti-Galectin 1 antibody (ab154351) at 1/10000 dilution

All lanes:

BCL-1 whole cell lysate at 30 µg

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LGALS1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P09382

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Primary Antibodies

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Proteins & Peptides

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Primary Antibodies

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Assay Kits

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.
Biological function summary

This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.

Pathways

The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.

Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed : 24752896).
See full target information LGALS1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 10:9172-9185 PubMed32802185

2020

Pancreatic Cancer detection via Galectin-1-targeted Thermoacoustic Imaging: validation in an heterozygosity model.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Qin,Baohua Qin,Chang Yuan,Qun Chen,Da Xing

Journal of virology 92: PubMed29899086

2018

Modulation of Cellular CpG DNA Methylation by Kaposi's Sarcoma-Associated Herpesvirus.

Applications

Unspecified application

Species

Unspecified reactive species

Guy Journo,Carmel Tushinsky,Asia Shterngas,Nir Avital,Yonatan Eran,Marcela Viviana Karpuj,Milana Frenkel-Morgenstern,Meir Shamay
View all publications
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