AB108389

Anti-Galectin 1 antibody [EPR3205]

Name: Anti-Galectin 1 antibody [EPR3205] (ab108389) | Abcam
Brand: Abcam Limited
SKU: AB108389
Price: 395 USD
Availability: InStock
Rating: 5 (1 reviews)

BOND RX™ Validated
RabMAb
Recombinant
What is this?

(1 Review)

(15 Publications)

Rabbit Recombinant Monoclonal Galectin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 15 publications.

View Alternative Names

Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Immunocytochemistry/immunofluorescence analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (AB108389)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling Galectin 1 with ab108389 at 1/3000 dilution (0.42 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody. Positive staining on vessels in human placenta tissue. The section was incubated with ab108389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 mins.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Immunocytochemistry/immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Unknown

Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389)

All lanes:

Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

Lane 1:

HL60 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Predicted band size: 15 kDa

false

Unknown

Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389)

All lanes:

Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution

Lane 1:

mouse heart tissue lysate at 10 µg

Lane 2:

mouse kidney tissue lysate at 10 µg

Lane 3:

mouse spleen tissue lysate at 10 µg

Lane 4:

rat heart tissue lysate at 10 µg

Lane 5:

rat kidney tissue lysate at 10 µg

Lane 6:

NIH 3T3 cell lysate at 10 µg

Predicted band size: 15 kDa

false

Carrier free

Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free
HRP

HRP Anti-Galectin 1 antibody [EPR3205]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Galectin 1 antibody [EPR3205]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Galectin 1 antibody [EPR3205]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Galectin 1 antibody [EPR3205]
660 APC

APC Anti-Galectin 1 antibody [EPR3205]
578 PE

PE Anti-Galectin 1 antibody [EPR3205]
Carrier free

Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (Detector)
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Galectin 1 antibody [EPR3205]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Galectin 1 antibody [EPR3205]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3205

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/3000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.

Biological function summary

This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.

Pathways

The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.

Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed : 24752896).

See full target information LGALS1

style

left-column

Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 42:207-225 PubMed39285911

2024

Versatile effects of galectin-1 protein-containing lipid bilayer coating for cardiovascular applications.

Applications

Unspecified application

Species

Unspecified reactive species

Md Lemon Hasan,Ju Ro Lee,Khandoker Asiqur Rahaman,Dae Hyeok Yang,Yoon Ki Joung

Oncology letters 25:271 PubMed37216162

2023

Snail inhibits metastasis via regulation of E‑cadherin and is associated with prognosis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weimin Wang,Jun Jin,Zhen Zhou,Yunfan Wang,Ke Min,Xin Zuo,Jiaping Jiang,Yan Zhou,Jun Shi

International journal of oncology 61: PubMed36177897

2022

Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Muhammad Ali,Yang Wang,Qian-Nan Sun,Xiao-Dong Zhu,Dong Tang,Wei Wang,Cang-Yuan Zhang,Hai-Hua Zhou,Dao-Rong Wang

Cancer immunology research 9:503-513 PubMed33771821

2021

Myeloma-Secreted Galectin-1 Potently Interacts with CD304 on Monocytic Myeloid-Derived Suppressor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Young Lim,Tae-Woo Kim,Da-Bin Ryu,Sung-Soo Park,Sung-Eun Lee,Byung-Soo Kim,Chang-Ki Min

Arteriosclerosis, thrombosis, and vascular biology 41:331-345 PubMed33147994

2020

Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Tsai Chiang,I-Ming Chen,Fu-Fei Hsu,Yen-Hui Chen,Min-Shao Tsai,Yaw-Wen Hsu,Hsin-Bang Leu,Po-Hsun Huang,Jaw-Wen Chen,Fu-Tong Liu,Ying-Hwa Chen,Lee-Young Chau

PLoS biology 16:e2006649 PubMed30550561

2018

CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-β.

Applications

Unspecified application

Species

Unspecified reactive species

Shima Shahbaz,Najmeh Bozorgmehr,Petya Koleva,Afshin Namdar,Juan Jovel,Roy A Fava,Shokrollah Elahi

Pediatric blood & cancer 65:e27229 PubMed29797641

2018

Both serum and tissue Galectin-1 levels are associated with adverse clinical features in neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Chen,Yuanxia Cai,Min Zhang,Zhixiang Wu,Yeming Wu

Cell death & disease 9:416 PubMed29549328

2018

Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gao,Xiaoju Li,Zhen Shu,Kuo Zhang,Xiaochang Xue,Weina Li,Qiang Hao,Zhaowei Wang,Wangqian Zhang,Shuning Wang,Cheng Zeng,Dong Fan,Wei Zhang,Yingqi Zhang,Huadong Zhao,Meng Li,Cun Zhang

Oncotarget 7:57099-57116 PubMed27494859

2016

MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yu You,Jia-Xin Tan,Hai-Su Dai,Hao-Wei Chen,Xue-Jun Xu,Ai-Gang Yang,Yu-Jun Zhang,Lian-Hua Bai,Ping Bie

Chembiochem : a European journal of chemical biology 16:2023-8 PubMed26296625

2015

Microdomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Leonhard Möckl,Andrea K Horst,Katharina Kolbe,Thisbe K Lindhorst,Christoph Bräuchle

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Galectin 1 antibody [EPR3205]

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise