Rabbit Recombinant Monoclonal Galectin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 13 publications.
Images
![Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--western-blot-img86826.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389)")
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--immunocytochemistry-immunofluorescence-img83231.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389)")
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--immunocytochemistry-immunofluorescence-img104393.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (AB108389)")
![Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--western-blot-img77477.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Galectin 1 antibody [EPR3205] (AB108389)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (AB108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405258.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (AB108389)")
Publications
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- Oncology letters 25:2712023Snail inhibits metastasis via regulation of E‑cadherin and is associated with prognosis in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeimin Wang,Jun Jin,Zhen Zhou,Yunfan Wang,Ke Min,Xin Zuo,Jiaping Jiang,Yan Zhou,Jun ShiPubMed 37216162
- Cancer immunology research 9:503-5132021Myeloma-Secreted Galectin-1 Potently Interacts with CD304 on Monocytic Myeloid-Derived Suppressor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Young Lim,Tae-Woo Kim,Da-Bin Ryu,Sung-Soo Park,Sung-Eun Lee,Byung-Soo Kim,Chang-Ki MinPubMed 33771821
- Arteriosclerosis, thrombosis, and vascular biology 41:331-3452020Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Tsai Chiang,I-Ming Chen,Fu-Fei Hsu,Yen-Hui Chen,Min-Shao Tsai,Yaw-Wen Hsu,Hsin-Bang Leu,Po-Hsun Huang,Jaw-Wen Chen,Fu-Tong Liu,Ying-Hwa Chen,Lee-Young ChauPubMed 33147994
- PLoS biology 16:e20066492018CD71+VISTA+ erythroid cells promote the development and function of regulatory T cells through TGF-β.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShima Shahbaz et. al.PubMed 30550561
- Pediatric blood & cancer 65:e272292018Both serum and tissue Galectin-1 levels are associated with adverse clinical features in neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Chen et. al.PubMed 29797641
- Cell death & disease 9:4162018Nuclear galectin-1-FOXP3 interaction dampens the tumor-suppressive properties of FOXP3 in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Gao et. al.PubMed 29549328
- Oncotarget 7:57099-571162016MiRNA-22 inhibits oncogene galectin-1 in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu You,Jia-Xin Tan,Hai-Su Dai,Hao-Wei Chen,Xue-Jun Xu,Ai-Gang Yang,Yu-Jun Zhang,Lian-Hua Bai,Ping BiePubMed 27494859
- Chembiochem : a European journal of chemical biology 16:2023-82015Microdomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycoproteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonhard Möckl,Andrea K Horst,Katharina Kolbe,Thisbe K Lindhorst,Christoph BräuchlePubMed 26296625
- PloS one 9:e955612014Identification of O-GlcNAc modification targets in mouse retinal pericytes: implication of p53 in pathogenesis of diabetic retinopathy.
- PloS one 9:e955292014Galactodendritic phthalocyanine targets carbohydrate-binding proteins enhancing photodynamic therapy.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/3000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
1 product for Alternative Version
Target data
Function
Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Alternative names
Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1
Recommended products
Rabbit Recombinant Monoclonal Galectin 1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 13 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR3205
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.
- Biological function summary
This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.
- Pathways
The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.
- Associated diseases and disorders
Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--western-blot-img86826.jpg "Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)")
Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
All lanes: Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution
Lane 1: HL60 cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Predicted band size: 15 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--immunocytochemistry-immunofluorescence-img83231.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)")
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
Immunocytochemistry/immunofluorescence analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--immunocytochemistry-immunofluorescence-img104393.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)")
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3205] (ab108389)
Immunocytochemistry/immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells labelling Galectin 1 with purified ab108389 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue).
![Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--western-blot-img77477.jpg "Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)")
Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389)
All lanes: Western blot - Anti-Galectin 1 antibody [EPR3205] (ab108389) at 1/1000 dilution
Lane 1: mouse heart tissue lysate at 10 µg
Lane 2: mouse kidney tissue lysate at 10 µg
Lane 3: mouse spleen tissue lysate at 10 µg
Lane 4: rat heart tissue lysate at 10 µg
Lane 5: rat kidney tissue lysate at 10 µg
Lane 6: NIH 3T3 cell lysate at 10 µg
Predicted band size: 15 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (ab108389), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-ab108389--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405258.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (ab108389)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] (ab108389)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling Galectin 1 with ab108389 at 1/3000 dilution (0.42 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. Positive staining on vessels in human placenta tissue. The section was incubated with ab108389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 mins.
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