Rabbit Recombinant Monoclonal Galectin 1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (AB271876), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-bsa-and-azide-free-ab271876--western-blot-img86826.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (AB271876)")
![Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (AB271876), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-bsa-and-azide-free-ab271876--western-blot-img77477.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (AB271876)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (AB271876), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-bsa-and-azide-free-ab271876--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405258.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (AB271876)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Expected | Tested | Not recommended |
| Mouse | Expected | Not recommended | Tested | Expected | Not recommended |
| Rat | Expected | Not recommended | Predicted | Predicted | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed:24752896).
Alternative names
Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1
Recommended products
Rabbit Recombinant Monoclonal Galectin 1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3205
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab271876 is the carrier-free version of Anti-Galectin 1 antibody [EPR3205] ab108389.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.
- Biological function summary
This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.
- Pathways
The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.
- Associated diseases and disorders
Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-bsa-and-azide-free-ab271876--western-blot-img86826.jpg "Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876)")
Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 1 antibody [EPR3205] ab108389).
All lanes: Western blot - Anti-Galectin 1 antibody [EPR3205] (Anti-Galectin 1 antibody [EPR3205] ab108389) at 1/1000 dilution
Lane 1: HL60 cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Predicted band size: 15 kDa
![Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-bsa-and-azide-free-ab271876--western-blot-img77477.jpg "Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876)")
Western blot - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 1 antibody [EPR3205] ab108389).
All lanes: Western blot - Anti-Galectin 1 antibody [EPR3205] (Anti-Galectin 1 antibody [EPR3205] ab108389) at 1/1000 dilution
Lane 1: mouse heart tissue lysate at 10 µg
Lane 2: mouse kidney tissue lysate at 10 µg
Lane 3: mouse spleen tissue lysate at 10 µg
Lane 4: rat heart tissue lysate at 10 µg
Lane 5: rat kidney tissue lysate at 10 µg
Lane 6: NIH 3T3 cell lysate at 10 µg
Predicted band size: 15 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr3205-bsa-and-azide-free-ab271876--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405258.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3205] - BSA and Azide free (ab271876)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labelling Galectin 1 with Anti-Galectin 1 antibody [EPR3205] ab108389 at 1/3000 dilution (0.42 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. Positive staining on vessels in human placenta tissue. The section was incubated with Anti-Galectin 1 antibody [EPR3205] ab108389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution1) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 1 antibody [EPR3205] ab108389).
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