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Rabbit Recombinant Monoclonal Galectin 1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

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Images

Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] (AB138513), expandable thumbnail
  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (AB138513), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] (AB138513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (AB138513), expandable thumbnail
  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (AB138513), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/16000

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

-

Species

Mouse

Dilution info

1/10000

Notes

-

Species

Rat

Dilution info

1/10000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Alternative names

Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1

Recommended products

Rabbit Recombinant Monoclonal Galectin 1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3206(2)

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.

Biological function summary

This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.

Pathways

The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.

Associated diseases and disorders

Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

    Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Galectin 1 with Purified ab138513 at 1:30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513), expandable thumbnail

    Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

    All lanes: Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/10000 dilution

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

    Lane 2: Mouse heart lysate at 15 µg

    Lane 3: Rat heart lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 15 kDa

    Observed band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

    Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Galectin 1 with Purified ab138513 at 1/50 dilution (5.4 µg/mL). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Galectin 1 with Purified ab138513 at 1/16000 dilution (0.02 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3

  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513), expandable thumbnail

    Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

    Lanes 1- 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab138513 was shown to react with Galectin 1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human LGALS1 (Galectin 1) knockout HEK-293T cell line ab266850 (knockout cell lysate Human LGALS1 (Galectin 1) knockout HEK-293T cell lysate ab256974) was used. Wild-type HEK-293T and LGALS1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab138513 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: LGALS1 knockout HEK-293T cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 15 kDa

    Observed band size: 15 kDa

  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513), expandable thumbnail

    Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513)

    Lanes 1 - 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab138513 was shown to specifically react with Galectin 1 in wild-type HAP1 cells as signal was lost in LGALS1 (Galectin 1) knockout cells. Wild-type and LGALS1 (Galectin 1) knockout samples were subjected to SDS-PAGE. ab138513 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: LGALS1 (Galectin 1) knockout HAP1 whole cell lysate at 20 µg

    Predicted band size: 15 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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