Rabbit Recombinant Monoclonal Galectin 1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--flow-cytometry-intracellular-img168014.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111)")
![Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--western-blot-img165899.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111)")
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--immunocytochemistry-immunofluorescence-img164908.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164654.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111)")
![Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--western-blot-img4866.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (AB240111)")
Publications
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- Cellular & molecular biology letters 28:402023Galectin-1 promotes angiogenesis and chondrogenesis during antler regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXunsheng Li,Wanwan Shi,Guanning Wei,Jinpeng Lv,Datao Wang,Baorui Xing,Jue Zhou,Jianwei Zhao,Hongmei SunPubMed 37189051
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected |
| Mouse | Predicted | Predicted | Predicted | Expected |
| Rat | Predicted | Predicted | Predicted | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
11 products for Alternative Product
3 products for Alternative Version
Target data
Function
Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Alternative names
Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1
Recommended products
Rabbit Recombinant Monoclonal Galectin 1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR3206(2)
- Purification technique
Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab240111 is the carrier-free version of Anti-Galectin 1 antibody [EPR3206(2)] ab138513.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.
- Biological function summary
This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.
- Pathways
The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.
- Associated diseases and disorders
Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--flow-cytometry-intracellular-img168014.jpg "Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)")
Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
This data was developed using ab240111, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling Galectin 1 with Purified ab240111 at 1:30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).![Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--western-blot-img165899.jpg "Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)")
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
All lanes: Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (Anti-Galectin 1 antibody [EPR3206(2)] ab138513) at 1/10000 dilution
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg
Lane 2: Mouse heart lysate at 15 µg
Lane 3: Rat heart lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 14 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--immunocytochemistry-immunofluorescence-img164908.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)")
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
This data was developed using Anti-Galectin 1 antibody [EPR3206(2)] ab138513, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Galectin 1 with Purified Anti-Galectin 1 antibody [EPR3206(2)] ab138513 at 1/50 dilution (5.4 µg/mL). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164654.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
This data was developed using ab240111, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Galectin 1 with Purified ab240111 at 1/16000 dilution (0.02 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3![Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111), expandable thumbnail](https://content.abcam.com/products/images/galectin-1-antibody-epr32062-bsa-and-azide-free-ab240111--western-blot-img4866.jpg "Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)")
Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
This data was developed using the same antibody clone in a different buffer formation (Anti-Galectin 1 antibody [EPR3206(2)] ab138513).
Lanes 1- 2: Merged signal (red and green). Green - Anti-Galectin 1 antibody [EPR3206(2)] ab138513 observed at 15 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-Galectin 1 antibody [EPR3206(2)] ab138513 was shown to react with Galectin 1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line Human LGALS1 (Galectin 1) knockout HEK-293T cell line ab266850 (knockout cell lysate Human LGALS1 (Galectin 1) knockout HEK-293T cell lysate ab256974) was used. Wild-type HEK-293T and LGALS1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Anti-Galectin 1 antibody [EPR3206(2)] ab138513 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Galectin 1 antibody [EPR3206(2)] (Anti-Galectin 1 antibody [EPR3206(2)] ab138513) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: LGALS1 knockout HEK-293T cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
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