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Rabbit Recombinant Monoclonal Galectin 1 antibody. C-terminal. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human samples.

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Images

Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (AB192842), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (AB192842), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (AB192842), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (AB192842), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (AB192842), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrFlow Cyt (Intra)
Human
Expected
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Primary antibody incubation for 1 hour at room temperature.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Primary antibody incubation for 10 minutes at room temperature.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Primary antibody incubation for 30 minutes at 4°C.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed:24752896).

Alternative names

Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1

Recommended products

Rabbit Recombinant Monoclonal Galectin 1 antibody. C-terminal. Suitable for WB, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP247

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Galectin-1 also referred to as GAL1 or galectin 1 is a protein with a molecular mass of approximately 14.5 kDa. It belongs to a group called the beta-galactoside-binding lectins and is typically characterized by its distinct carbohydrate-recognition domain. Galectin-1 is expressed in a variety of tissues including muscle epithelial cells the nervous system and immune cells. In particular high expression levels are found in the placenta and the thymus indicating a role in development and immune function.

Biological function summary

This protein mediates a variety of cellular processes such as cell differentiation apoptosis and immune cell modulation. As a monomer galectin-1 binds specifically to beta-galactoside residues on the cell surface. It plays a significant role in regulation of cell signaling circuits affecting T-cell activation as well as macrophage function. The Galectin-1 protein does not commonly form complexes but can influence interactions of other proteins by binding to glycoproteins on the cell surface which can modulate signaling outcomes.

Pathways

The protein is actively involved in pathways related to cell-cell interactions and immune modulation. It interacts with pathways like Ras signaling which is fundamental in cell growth proliferation and survival. Through these pathways galectin-1 is closely related to other proteins including Ras and Raf which are critical in transmitting signals from the cell surface to the DNA in the nucleus affecting gene expression involved in apoptosis and autophagy.

Associated diseases and disorders

Galectin-1 association with cancer progression and autoimmune diseases reflects its role in immune system regulation. High galectin-1 levels have been recorded in various tumors where the protein promotes tumor progression by supporting angiogenesis and suppressing immune response against cancer cells. In autoimmune disorders such as multiple sclerosis modulation of Galectin-1 levels can alter disease progression and the immune system’s behavior. The protein interacts with other key regulators like cytokines emphasizing its importance in immune-inflammatory pathways and potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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5 product images

  • Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842), expandable thumbnail

    Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    All lanes: Western blot - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842) at 1/100 dilution

    All lanes: NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for Galectin 1 using ab192842 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    IHC image of Galectin 1 staining in a section of frozen normal human placenta* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab192842, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    IHC image of Galectin 1 staining in a section of frozen normal human prostate carcinoma* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab192842, 10ugml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 1 antibody [SP247] - C-terminal (ab192842)

    Intracellular flow cytometric analysis ofHL-60 (Human promyelocytic leukemia cell line) cells labeling Galectin 1 with ab192842 at 1/100 dilution (green) compared to a negative control of rabbit IgG (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com