Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261]

14 product images

• 

  Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the higher MW band at approximately 50 kDa (in lanes 4 and lane 7) is unknown.

  Exposure time: Lanes 1 and 5: 59 seconds, Lanes 2-4 and 6-8 180 seconds

  All lanes: Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution

  Lane 1: Mouse placenta tissue lysate at 20 µg

  Lane 2: Mouse kidney tissue lysate at 20 µg

  Lane 3: Mouse spleen tissue lysate at 20 µg

  Lane 4: Mouse thymus tissue lysate at 20 µg

  Lane 5: Rat placenta tissue lysate at 20 µg

  Lane 6: Rat kidney tissue lysate at 20 µg

  Lane 7: Rat spleen tissue lysate at 20 µg

  Lane 8: Rat thymus tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 15 kDa, 30 kDa, 40 kDa

• 

  Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: Neuro-2a is the low expression cell of Galectin 3.

  The identity of the higher MW band at approximately 50 kDa (in lanes 6-8) is unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution

  Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

  Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

  Lane 3: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

  Lane 4: C2C12 (mouse myoblast) whole cell lysate at 20 µg

  Lane 5: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

  Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 7: Human placenta tissue lysates at 20 µg

  Lane 8: Human colon tissue lysates at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 15 kDa, 30 kDa, 40 kDa, 36 kDa

  Exposure time: 59s

• 

  Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

• 

  Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

• 

  Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

  Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

• 

  Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Low expression: LNCAP is the low expression cell of Galectin 1 and Galectin 3 (PMID: 9917495).

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution

  Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

  Lane 2: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 4: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 5: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

  Lane 6: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 15 kDa, 30 kDa, 40 kDa, 36 kDa

  Exposure time: 37s

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and nuclear staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and nuclear staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and nuclear staining in C2C12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human gastric carcinoma. The section was incubated with ab322347 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human kidney. The section was incubated with ab322347 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on human spleen. The section was incubated with ab322347 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 (1.02 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse spleen. The section was incubated with ab322347 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

  Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 (1.02 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat spleen. The section was incubated with ab322347 for 30 mins at room temperature.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins