Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261]
- 20ul selling size
- BOND RX™ Validated
- RabMAb
- Recombinant
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Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] is a Rabbit Recombinant Multiclonal antibody that is used in Galectin 1 + Galectin 3 + Galectin 8 Flow Cytometry (Intra), ICC/IF, IHC-P, Western Blot. Suitable for Human, Mouse, Rat samples.
Galectins are β-galactoside-binding proteins that play multifaceted roles in cancer progression, including tumor cell proliferation, angiogenesis, immune evasion, and metastasis. They are critical targets for therapeutic intervention, with research focusing on galectin inhibitors to enhance cancer treatment efficacy and overcome resistance to various anticancer therapies.
View Alternative Names
Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1, MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP, Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, Po66-CBP, PCTA-1, LGALS8, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, IgE-binding protein, L-34 galactoside-binding lectin, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, Lgals3, Galectin-8, Gal-8, LGALS-8, Lgals8, Gbp, Lgals1, Galectin-1, Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, S-Lac lectin 1, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, IgE-binding protein, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, Lgals3, Galectin-1, Gal-1, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, RL 14.5, S-Lac lectin 1, Galectin-8, Gal-8, 30 kDa S-type lectin, RL-30
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human gastric carcinoma. The section was incubated with ab322347 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human kidney. The section was incubated with ab322347 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human spleen. The section was incubated with ab322347 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 (1.02 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with ab322347 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in C2C12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 (1.02 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen. The section was incubated with ab322347 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
- WB
Supplier Data
Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Low expression : LNCAP is the low expression cell of Galectin 1 and Galectin 3 (PMID : 9917495).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution
Lane 1:
K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
Lane 2:
HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3:
LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5:
THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 6:
HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 15 kDa,30 kDa,40 kDa,36 kDa
false
Exposure time: 37s
- WB
Supplier Data
Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Low expression : Neuro-2a is the low expression cell of Galectin 3.
The identity of the higher MW band at approximately 50 kDa (in lanes 6-8) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution
Lane 1:
PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 2:
C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Lane 3:
L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg
Lane 4:
C2C12 (mouse myoblast) whole cell lysate at 20 µg
Lane 5:
RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 6:
Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 7:
Human placenta tissue lysates at 20 µg
Lane 8:
Human colon tissue lysates at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 15 kDa,30 kDa,40 kDa,36 kDa
false
Exposure time: 59s
- WB
Supplier Data
Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
The identity of the higher MW band at approximately 50 kDa (in lanes 4 and lane 7) is unknown.
Exposure time : Lanes 1 and 5 : 59 seconds, Lanes 2-4 and 6-8 180 seconds
All lanes:
Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution
Lane 1:
Mouse placenta tissue lysate at 20 µg
Lane 2:
Mouse kidney tissue lysate at 20 µg
Lane 3:
Mouse spleen tissue lysate at 20 µg
Lane 4:
Mouse thymus tissue lysate at 20 µg
Lane 5:
Rat placenta tissue lysate at 20 µg
Lane 6:
Rat kidney tissue lysate at 20 µg
Lane 7:
Rat spleen tissue lysate at 20 µg
Lane 8:
Rat thymus tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 15 kDa,30 kDa,40 kDa
false
Reactivity data
Product details
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
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Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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