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Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] is a Rabbit Recombinant Multiclonal antibody that is used in Galectin 1 + Galectin 3 + Galectin 8 Flow Cytometry (Intra), ICC/IF, IHC-P, Western Blot. Suitable for Human, Mouse, Rat samples.

Galectins are β-galactoside-binding proteins that play multifaceted roles in cancer progression, including tumor cell proliferation, angiogenesis, immune evasion, and metastasis. They are critical targets for therapeutic intervention, with research focusing on galectin inhibitors to enhance cancer treatment efficacy and overcome resistance to various anticancer therapies.

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Images

Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347), expandable thumbnail
  • Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (AB322347), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWBFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Tested
Tested
Tested
Tested
Not recommended
Rat
Tested
Tested
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Species
Rat
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis. Plays a negative role in Th17 cell differentiation via activation of the receptor CD69 (PubMed:24752896).

Additional Targets

LGALS3, LGALS8, Lgals3, Lgals8, Lgals1, Lgals3, Lgals8, Lgals1

Alternative names

Galectin-1, Gal-1, 14 kDa laminin-binding protein, 14 kDa lectin, Beta-galactoside-binding lectin L-14-I, Galaptin, HBL, HPL, Lactose-binding lectin 1, Lectin galactoside-binding soluble 1, Putative MAPK-activating protein PM12, S-Lac lectin 1, HLBP14, LGALS1

Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] is a Rabbit Recombinant Multiclonal antibody that is used in Galectin 1 + Galectin 3 + Galectin 8 Flow Cytometry (Intra), ICC/IF, IHC-P, Western Blot. Suitable for Human, Mouse, Rat samples.

Galectins are β-galactoside-binding proteins that play multifaceted roles in cancer progression, including tumor cell proliferation, angiogenesis, immune evasion, and metastasis. They are critical targets for therapeutic intervention, with research focusing on galectin inhibitors to enhance cancer treatment efficacy and overcome resistance to various anticancer therapies.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM1261
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

  • Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the higher MW band at approximately 50 kDa (in lanes 4 and lane 7) is unknown.

    Exposure time: Lanes 1 and 5: 59 seconds, Lanes 2-4 and 6-8 180 seconds

    All lanes: Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution

    Lane 1: Mouse placenta tissue lysate at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: Mouse thymus tissue lysate at 20 µg

    Lane 5: Rat placenta tissue lysate at 20 µg

    Lane 6: Rat kidney tissue lysate at 20 µg

    Lane 7: Rat spleen tissue lysate at 20 µg

    Lane 8: Rat thymus tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 15 kDa, 30 kDa, 40 kDa

  • Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Neuro-2a is the low expression cell of Galectin 3.

    The identity of the higher MW band at approximately 50 kDa (in lanes 6-8) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution

    Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Lane 3: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

    Lane 4: C2C12 (mouse myoblast) whole cell lysate at 20 µg

    Lane 5: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 6: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 7: Human placenta tissue lysates at 20 µg

    Lane 8: Human colon tissue lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 15 kDa, 30 kDa, 40 kDa, 36 kDa

    Exposure time: 59s

  • Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblast) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: LNCAP is the low expression cell of Galectin 1 and Galectin 3 (PMID: 9917495).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 6: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 15 kDa, 30 kDa, 40 kDa, 36 kDa

    Exposure time: 37s

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in THP-1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 (mouse myoblast) cells labelling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/100 (5.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic and nuclear staining in C2C12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human gastric carcinoma. The section was incubated with ab322347 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human kidney. The section was incubated with ab322347 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/2000 (0.255 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human spleen. The section was incubated with ab322347 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 (1.02 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen. The section was incubated with ab322347 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 + Galectin 3 + Galectin 8 antibody [RM1261] (ab322347)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Galectin 1 + Galectin 3 + Galectin 8 with ab322347 at 1/500 (1.02 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat spleen. The section was incubated with ab322347 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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