Rabbit Polyclonal Galectin 10 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CLC.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CLC. Database link Q05315
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
Target data
Function
Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).
Alternative names
LGALS10, LGALS10A, CLC, Galectin-10, Gal-10, Charcot-Leyden crystal protein, Eosinophil lysophospholipase, Lysolecithin acylhydrolase
Recommended products
Rabbit Polyclonal Galectin 10 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CLC.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.12% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CLC. Database link Q05315
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-10 also known as Charcot-Leyden crystal protein is part of the galectin family of proteins. It functions by binding to carbohydrates due to its unique lectin properties. With a molecular mass of approximately 16.5 kDa this protein is mainly expressed in eosinophils and basophils cells that are significant in the immune system. Galectin-10 is secreted by these cells and also stored in their granules suggesting its importance in immune responses.
- Biological function summary
Galectin-10 influences cellular processes like apoptosis cell adhesion and immune cell migration contributing to immune regulation. It is not well known to form part of large complexes but its release has implications on cell signaling and tissue remodeling. The presence of galectin-10 in eosinophils suggests a role in inflammation given their involvement in allergic responses and asthma.
- Pathways
Studies associate galectin-10 with the allergy response and eosinophil apoptosis pathways. This protein cooperates with other galectin family members like galectin-3 in modulating cellular responses. Signaling interactions of galectin-10 impact pathways involving cell survival and death highlighting its role in immune system dynamics.
- Associated diseases and disorders
Researchers link galectin-10 to conditions such as asthma and eosinophilic disorders. Eosinophil activity in these diseases often correlates with elevated galectin-10 levels in tissues and bloodstream. Connections exist between galectin-10 and other inflammation-related proteins like interleukins influencing disease progression and severity through their regulatory roles in immune responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Galectin 10 antibody (ab231964)
All lanes: Western blot - Anti-Galectin 10 antibody (ab231964) at 2 µg/mL
All lanes: Recombinant human Galectin 10 protein
SecondaryAll lanes: HRP-Linked Guinea pig anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 16 kDa
Western blot - Anti-Galectin 10 antibody (ab231964)
All lanes: Western blot - Anti-Galectin 10 antibody (ab231964) at 2 µg/mL
All lanes: Human leukocyte cell lysate
SecondaryAll lanes: HRP-Linked Guinea pig anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody (ab231964)
Formalin-fixed, paraffin-embedded human stomach tissue stained for Galectin 10 using ab231964 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody (ab231964)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Galectin 10 using ab231964 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody (ab231964)
Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for Galectin 10 using ab231964 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody (ab231964)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Galectin 10 using ab231964 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody (ab231964)
Formalin-fixed, paraffin-embedded human cerebrum tissue stained for Galectin 10 using ab231964 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody (ab231964)
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Galectin 10 using ab231964 at 30 μg/ml in immunohistochemical analysis. DAB staining.
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