AB249338

Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Galectin 10 antibody. Carrier free. Suitable for ICC/IF, IHC-P, WB and reacts with Human samples.

View Alternative Names

LGALS10, LGALS10A, CLC, Galectin-10, Gal-10, Charcot-Leyden crystal protein, Eosinophil lysophospholipase, Lysolecithin acylhydrolase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

This data was developed using ab157475, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Galectin 10 with ab157475 at 1/40000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human tonsil. is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

The section was incubated with ab157475 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

This data was developed using ab157475, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labelling Galectin 10 with ab157475 at 1/40000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human colon carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

The section was incubated with ab157475 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument.

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

This data was developed using ab157475, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of THP-1 cells labeling Galectin 10 with ab157475 at 1/100 dilution.

Unknown

Western blot - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

This data was developed using ab157475, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Galectin 10 antibody [EPR11197] (<a href='/en-us/products/primary-antibodies/galectin-10-antibody-epr11197-ab157475'>ab157475</a>) at 1/10000 dilution

Lane 1:

Human spleen lysate at 10 µg

Lane 2:

Human bone marrow lysate at 10 µg

Lane 3:

Human tonsil lysate at 10 µg

Lane 4:

THP-1 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 16 kDa

false

Unconjugated

Anti-Galectin 10 antibody [EPR11197]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11197

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249338 is the carrier-free version of ab157475.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-10 also known as Charcot-Leyden crystal protein is part of the galectin family of proteins. It functions by binding to carbohydrates due to its unique lectin properties. With a molecular mass of approximately 16.5 kDa this protein is mainly expressed in eosinophils and basophils cells that are significant in the immune system. Galectin-10 is secreted by these cells and also stored in their granules suggesting its importance in immune responses.

Biological function summary

Galectin-10 influences cellular processes like apoptosis cell adhesion and immune cell migration contributing to immune regulation. It is not well known to form part of large complexes but its release has implications on cell signaling and tissue remodeling. The presence of galectin-10 in eosinophils suggests a role in inflammation given their involvement in allergic responses and asthma.

Pathways

Studies associate galectin-10 with the allergy response and eosinophil apoptosis pathways. This protein cooperates with other galectin family members like galectin-3 in modulating cellular responses. Signaling interactions of galectin-10 impact pathways involving cell survival and death highlighting its role in immune system dynamics.

Researchers link galectin-10 to conditions such as asthma and eosinophilic disorders. Eosinophil activity in these diseases often correlates with elevated galectin-10 levels in tissues and bloodstream. Connections exist between galectin-10 and other inflammation-related proteins like interleukins influencing disease progression and severity through their regulatory roles in immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).

See full target information CLC

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

Western blot - Anti-Galectin 10 antibody [EPR11197] - BSA and Azide free (AB249338)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise