AB278071

Anti-Galectin 3 antibody [5C21]

Name: Anti-Galectin 3 antibody [5C21] (ab278071) | Abcam
Brand: Abcam Limited
SKU: AB278071
Price: 515 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Galectin 3 antibody. Suitable for ICC/IF, WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LGALS3.

View Alternative Names

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [5C21] (AB278071)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [5C21] (AB278071)

Cortical neuron-glial cell culture from E20 rat stained for galectin 3 (red) using ab278071 at 1/1000 dilution in immunocytochemistry. A chicken polyclonal antibody to MAP2 (green) was used at 1/10000 dilution. The blue is Hoechst staining of nuclear DNA.

Supplier Data

Western blot - Anti-Galectin 3 antibody [5C21] (AB278071)

The band at about 30kDa mark corresponds to the galectin 3 protein. The same blot was simultaneously probed with chicken polyclonal to HSP60 (red), at 1/20000 dilution which reveals HSP60 protein in all preparations.

Lane 1:

Western blot - Anti-Galectin 3 antibody [5C21] (ab278071)

Lanes 2 - 8:

Western blot - Anti-Galectin 3 antibody [5C21] (ab278071) at 1/2000 dilution

Lane 1:

Molecular weight marker

Lane 2:

Mouse brain lysate

Lane 3:

Rat brain

Lane 4:

Rat liver

Lane 5:

Rat heart

Lane 6:

Rat kidney

Lane 7:

Rat lung

Lane 8:

HEK-293 cell lysate

Predicted band size: 26 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5C21

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human LGALS3.

P17931

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from Tissue Culture supernatant.

Storage buffer

Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.

Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

See full target information LGALS3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neurochemical research 50:180 PubMed40471451

2025

miR-210 Regulates Autophagy Through the AMPK/mTOR Signaling Pathway, Reduces Neuronal Cell Death and Inflammatory Responses, and Enhances Functional Recovery Following Cerebral Hemorrhage in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Wang,Lei Jiang,Jin-Jie Tian,Lin-Lin Zhu,He-Jun Dai,Chao Guo,Ling-Yun Zhou,Lei Wang,Yong Lu,Yi Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Galectin 3 antibody [5C21]

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [5C21] (AB278071)

Western blot - Anti-Galectin 3 antibody [5C21] (AB278071)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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