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Rabbit Polyclonal Galectin 3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS3.


Images

Western blot - Anti-Galectin 3 antibody (AB227249), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody (AB227249), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody (AB227249), expandable thumbnail
  • Western blot - Anti-Galectin 3 antibody (AB227249), expandable thumbnail
  • Western blot - Anti-Galectin 3 antibody (AB227249), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human LGALS3. The exact immunogen used to generate this antibody is proprietary information. Database link P17931

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Rat
Tested
Expected
Expected
Rhesus monkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/5000.00000 - 1/20000.00000

Notes

-

Species

Human

Dilution info

1/5000.00000 - 1/20000.00000

Notes

-

Predicted
Predicted

Species

Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rhesus monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Alternative names

Recommended products

Rabbit Polyclonal Galectin 3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS3.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human LGALS3. The exact immunogen used to generate this antibody is proprietary information. Database link P17931
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.

Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Associated diseases and disorders

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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6 product images

  • Western blot - Anti-Galectin 3 antibody (ab227249), expandable thumbnail

    Western blot - Anti-Galectin 3 antibody (ab227249)

    All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution

    Lane 1: WT HeLa cell extract at 30 µg

    Lane 2: Galectin 3 Knockout HeLa cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody (ab227249), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody (ab227249)

    Paraffin-embedded HBL435 xenograft tissue stained for Galectin 3 using ab227249 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody (ab227249), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody (ab227249)

    Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Galectin 3 (green) using ab227249 at 1/1000 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • Western blot - Anti-Galectin 3 antibody (ab227249), expandable thumbnail

    Western blot - Anti-Galectin 3 antibody (ab227249)

    12% SDS-PAGE gel.

    All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution

    Lane 1: A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

    Lane 2: H1299 whole cell lysate at 30 µg

    Lane 3: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

    Lane 4: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 26 kDa

  • Western blot - Anti-Galectin 3 antibody (ab227249), expandable thumbnail

    Western blot - Anti-Galectin 3 antibody (ab227249)

    12% SDS PAGE gel-

    All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution

    Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Lane 2: Galectin 3 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Predicted band size: 26 kDa

  • Western blot - Anti-Galectin 3 antibody (ab227249), expandable thumbnail

    Western blot - Anti-Galectin 3 antibody (ab227249)

    12 % SDS-PAGE gel.

    All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution

    All lanes: Rat colon lysate at 50 µg

    Predicted band size: 26 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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