Rabbit Polyclonal Galectin 3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS3.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Rat | Tested | Expected | Expected |
Rhesus monkey | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
Select an associated product type
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
MAC2, LGALS3, MAC2, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP
Rabbit Polyclonal Galectin 3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS3.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.
Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.
Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1: WT HeLa cell extract at 30 µg
Lane 2: Galectin 3 Knockout HeLa cell extracts at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 26 kDa
Paraffin-embedded HBL435 xenograft tissue stained for Galectin 3 using ab227249 at 1/500 dilution in immunohistochemical analysis.
Ice-cold methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for Galectin 3 (green) using ab227249 at 1/1000 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
12% SDS-PAGE gel.
All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg
Lane 4: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS PAGE gel-
All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Lane 2: Galectin 3 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 26 kDa
12 % SDS-PAGE gel.
All lanes: Western blot - Anti-Galectin 3 antibody (ab227249) at 1/10000 dilution
All lanes: Rat colon lysate at 50 µg
Predicted band size: 26 kDa
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