AB53082

Anti-Galectin 3 antibody

Name: Anti-Galectin 3 antibody (ab53082) | Abcam
Brand: Abcam Limited
SKU: AB53082
Price: 435 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(39 Publications)

Rabbit Polyclonal Galectin 3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Synthetic Peptide within Human LGALS3.

View Alternative Names

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody (AB53082)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody (AB53082)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53082 at 1/50 dilution, with and without blocking peptide (ab53082 immunogen).

Unknown

Western blot - Anti-Galectin 3 antibody (AB53082)

All lanes:

Western blot - Anti-Galectin 3 antibody (ab53082) at 1/500 dilution

Lane 1:

Hela cell extract

Lane 2:

Hela cell extract with blocking peptide (ab53082 immunogen)

Predicted band size: 26 kDa

Observed band size: 26 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LGALS3. The exact immunogen used to generate this antibody is proprietary information.

P17931

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.

Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

See full target information LGALS3

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 30:261 PubMed39707203

2024

Endothelial-specific deletion of connexin 43 improves renal function and structure after acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Magali Genest,Satoshi Kinugasa,Elena Roger,Louis Boutin,Sandrine Placier,Stefanny Figueroa,Aude Dorison,Safia Hadjadj,Ines Baba,Emmanuel L Gautier,Panagiotis Kavvadas,Christos Chatziantoniou,Christos E Chadjichristos

Journal of clinical medicine 11: PubMed36362749

2022

The Expression of IL-1β Correlates with the Expression of Galectin-3 in the Tissue at the Maternal-Fetal Interface during the Term and Preterm Labor.

Applications

Unspecified application

Species

Unspecified reactive species

Nikola Jovic,Marija Milovanovic,Jovana Joksimovic Jovic,Marija Bicanin Ilic,Dejana Rakic,Vladimir Milenkovic,Bojana Stojanovic,Jelena Milovanovic,Aleksandar Arsenijevic,Nebojsa Arsenijevic,Mirjana Varjacic

The Journal of biological chemistry 298:101743 PubMed35183508

2022

Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3.

Applications

Unspecified application

Species

Unspecified reactive species

Mohit P Mathew,Lara K Abramowitz,Julie G Donaldson,John A Hanover

Journal of translational medicine 20:1 PubMed34980160

2022

Bilirubin ameliorates murine atherosclerosis through inhibiting cholesterol synthesis and reshaping the immune system.

Applications

Unspecified application

Species

Unspecified reactive species

Guanmei Wen,Leyi Yao,Yali Hao,Jinheng Wang,Jinbao Liu

Frontiers in pharmacology 12:714683 PubMed34803672

2021

Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.

Applications

Unspecified application

Species

Unspecified reactive species

Nemanja Jovicic,Ivica Petrovic,Nada Pejnovic,Biljana Ljujic,Marina Miletic Kovacevic,Sladjana Pavlovic,Ilija Jeftic,Aleksandar Djukic,Ivan Srejovic,Vladimir Jakovljevic,Miodrag L Lukic

Journal of immunology (Baltimore, Md. : 1950) 207:65-76 PubMed34135057

2021

Impaired TRIM16-Mediated Lysophagy in Chronic Obstructive Pulmonary Disease Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Araya,Nayuta Saito,Yusuke Hosaka,Akihiro Ichikawa,Tsukasa Kadota,Yu Fujita,Shunsuke Minagawa,Hiromichi Hara,Shota Fujimoto,Hironori Kawamoto,Naoaki Watanabe,Akihiko Ito,Keitaro Okuda,Hanae Miyagawa,Junko Watanabe,Daisuke Takekoshi,Hirofumi Utsumi,Masahiro Yoshida,Mitsuo Hashimoto,Hiroshi Wakui,Saburo Ito,Takanori Numata,Shohei Mori,Hideki Matsudaira,Jun Hirano,Takashi Ohtsuka,Katsutoshi Nakayama,Kazuyoshi Kuwano

Cancers 13: PubMed34203725

2021

Diagnostic Value of Galectin-3 in Distinguishing Invasive Encapsulated Carcinoma from Noninvasive Follicular Thyroid Neoplasms with Papillary-Like Nuclear Features (NIFTP).

Applications

Unspecified application

Species

Unspecified reactive species

Guodong Fu,Olena Polyakova,Ronald S Chazen,Jeremy L Freeman,Ian J Witterick

BMC musculoskeletal disorders 22:494 PubMed34049522

2021

Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Shao Cheng,Kanghui Sun,Jing Wang,Xinhua Liu,Yongjian Zhao,Junjie Yang,Dongfeng Zhao,Chunchun Xue,Yuren Tao,Shitian Zhao,Hao Zhang,Sheng Lu,Qi Shi,Yongjun Wang,Bing Shu

Frontiers in pharmacology 12:588587 PubMed33953667

2021

Galectin-3 Mediated Inflammatory Response Contributes to Neurological Recovery by QiShenYiQi in Subacute Stroke Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yule Wang,Shuang He,Xinyan Liu,Zhixiong Li,Lin Zhu,Guangxu Xiao,Xiaoli Du,Hongxia Du,Wen Zhang,Yiqian Zhang,John Orgah,Yuxin Feng,Boli Zhang,Yan Zhu

Scientific reports 11:2613 PubMed33510341

2021

Inhibition of the CXCL9-CXCR3 axis suppresses the progression of experimental apical periodontitis by blocking macrophage migration and activation.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuya Hasegawa,V Venkata Suresh,Yoshio Yahata,Masato Nakano,Shigeto Suzuki,Shigeki Suzuki,Satoru Yamada,Hideki Kitaura,Itaru Mizoguchi,Yuichiro Noiri,Keisuke Handa,Masahiro Saito

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Galectin 3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody (AB53082)

Western blot - Anti-Galectin 3 antibody (AB53082)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

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