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Rabbit Recombinant Monoclonal Galectin 3 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.


Images

Western blot - Anti-Galectin 3 antibody [EPR2774] (AB76466), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Galectin 3 antibody [EPR2774] (AB76466), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [EPR2774] (AB76466), expandable thumbnail
  • Western blot - Anti-Galectin 3 antibody [EPR2774] (AB76466), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Tested
Rat
Not recommended
Predicted
Not recommended
Predicted
Predicted

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

10 products for Alternative Product

Target data

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Alternative names

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP

Recommended products

Rabbit Recombinant Monoclonal Galectin 3 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2774
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.

Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Associated diseases and disorders

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Galectin 3 antibody [EPR2774] (ab76466), expandable thumbnail

    Western blot - Anti-Galectin 3 antibody [EPR2774] (ab76466)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: Galectin 3 (KO) knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: MCF7 whole cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab76466 observed at 35 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab76466 was shown to specifically react with Galectin 3 (KO) when Galectin 3 (KO) knockout samples were used. Wild-type and Galectin 3 (KO) knockout samples were subjected to SDS-PAGE. ab76466 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.


    All lanes: Western blot - Anti-Galectin 3 antibody [EPR2774] (ab76466)

    Predicted band size: 26 kDa

    Observed band size: 35 kDa

  • Flow Cytometry (Intracellular) - Anti-Galectin 3 antibody [EPR2774] (ab76466), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 3 antibody [EPR2774] (ab76466)

    Overlay histogram showing THP1 cells stained with ab76466 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76466, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [EPR2774] (ab76466), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [EPR2774] (ab76466)

    ab76466, at a 1/100 dilution, staining Galectin 3 in HeLa cells by Immunofluorescence.

  • Western blot - Anti-Galectin 3 antibody [EPR2774] (ab76466), expandable thumbnail

    Western blot - Anti-Galectin 3 antibody [EPR2774] (ab76466)

    All lanes: Western blot - Anti-Galectin 3 antibody [EPR2774] (ab76466) at 1/5000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: SW480 cell lysate at 10 µg

    Lane 3: A431 cell lysate at 10 µg

    Lane 4: MCF7 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 26 kDa

    Observed band size: 30 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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