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AB317216

Anti-Galectin 3 antibody [HL2397]

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Rabbit Monoclonal Galectin 3 antibody. Suitable for sELISA, WB and reacts with Recombinant full length protein - Human, Human, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human LGALS3.

View Alternative Names

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP

5 Images
Western blot - Anti-Galectin 3 antibody [HL2397] (AB317216)
  • WB

Supplier Data

Western blot - Anti-Galectin 3 antibody [HL2397] (AB317216)

Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317216 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Galectin 3 antibody [HL2397] (ab317216) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

Galectin 3 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Galectin 3 antibody [HL2397] (AB317216)
  • WB

Supplier Data

Western blot - Anti-Galectin 3 antibody [HL2397] (AB317216)

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE and the membrane was blotted with ab317216 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Galectin 3 antibody [HL2397] (ab317216) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Sandwich ELISA - Anti-Galectin 3 antibody [HL2397] (AB317216)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Galectin 3 antibody [HL2397] (AB317216)

Sandwich ELISA detection of recombinant E.coli expressed full-length Galectin 3 protein. Capture : ab317216 (5 ug/mL). Detection : HRP-conjugated Galectin 3 antibody [HL2396] (1 ug/mL).

Sandwich ELISA - Anti-Galectin 3 antibody [HL2397] (AB317216)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Galectin 3 antibody [HL2397] (AB317216)

Sandwich ELISA detection of recombinant E.coli expressed full-length Galectin 3 protein. Capture : Galectin 3 antibody [HL2396] (5 ug/mL). Detection : HRP-conjugated ab317216 (1 ug/mL). Please notice that ab317216 needs to be conjugated to HRP to function as the detection antibody when paired with this capture antibody.

Western blot - Anti-Galectin 3 antibody [HL2397] (AB317216)
  • WB

Supplier Data

Western blot - Anti-Galectin 3 antibody [HL2397] (AB317216)

Whole cell extract (30 ug) was separated by 12% SDS-PAGE and the membrane was blotted with ab317216 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Galectin 3 antibody [HL2397] (ab317216) at 1/5000 dilution

All lanes:

MDCK whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2397

Isotype

IgG

Carrier free

No

Reacts with

Human, Dog

Applications

WB, sELISA

applications

Immunogen

Recombinant Fragment Protein within Human LGALS3.

P17931

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Dog": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
See full target information LGALS3
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Product promise

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