AB206435

Anti-Galectin 7 antibody [EPR19903]

Name: Anti-Galectin 7 antibody [EPR19903] (ab206435) | Abcam
Brand: Abcam Limited
SKU: AB206435
Price: 355 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal Galectin 7 antibody. Suitable for IP, WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

View Alternative Names

PIG1, LGALS7B, LGALS7, Galectin-7, Gal-7, HKL-14, PI7, p53-induced gene 1 protein

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of human skin is observed (PMID : 23658821). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR19903] (AB206435)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (Human keratinocyte cell line) cells labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HACAT cell line. The nuclear counterstain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : No staining in human kidney (PMID : 9697310).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemical analysis of paraffin-embedded human squamous carcinoma of the cervix tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on tumor cells of squamous carcinoma of the cervix is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HaCaT (Human keratinocyte cell line) cells labeling Galectin 7with ab206435 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Galectin 7 was immunoprecipitated from 0.35 mg of human skin lysate with ab206435 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab206435 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human skin lysate 10μg (Input).

Lane 2 : ab206435 IP in human skin lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab206435 in human skin lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Galectin 7 antibody [EPR19903] (ab206435)

Predicted band size: 15 kDa

false

Immunohistochemistry (Frozen sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat skin tissue labeling Galectin 7 with ab206435 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on epithelium of rat skin is observed. Nuclear counterstain : DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control : No staining on mouse stomach (PMID : 9697310).

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of rat skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Galectin 7 with ab206435 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on epithelium of mouse skin is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Blocking/Dilution buffer : 5% NFDM/TBST.

Galectin 7 is specifically expressed in stratified squamous epithelium, but not in non-epithelial organs such as skeletal muscle. PMID : 9697310; PMID : 7729568.

All lanes:

Western blot - Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/2000 dilution

Lane 1:

Human skin lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 14 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1/2 : 30 seconds; Lane 3 : 15 seconds.

Galectin 7 is specifically expressed in stratified squamous epithelium, but not in non-epithelial organs. PMID : 9697310; PMID : 7729568.

Lanes 1 - 2:

Western blot - Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/2000 dilution

Lane 3:

Western blot - Anti-Galectin 7 antibody [EPR19903] (ab206435) at 1/1000 dilution

Lane 1:

Rat skin lysate at 10 µg

Lane 2:

Mouse skin lysate at 10 µg

Lane 3:

HaCaT (Human keratinocyte cell line) whole cell lysate at 10 µg

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Lane 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 14 kDa

false

Carrier free

Anti-Galectin 7 antibody [EPR19903] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19903

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, WB, Flow Cyt (Intra), ICC/IF, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "(<a href='/en-us/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a>)" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "(<a href='/en-us/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a>)" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "(<a href='/en-us/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a>)" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 7 also known as Gal-7 is a member of the galectin family of beta-galactoside-binding proteins. It has a molecular mass of approximately 15 kDa. Structurally Galectin 7 has a single carbohydrate recognition domain (CRD) which allows it to bind glycoconjugates. Galectin 7 expresses in various tissues with high expression levels in the epidermis and in stratified squamous epithelia.

Biological function summary

Galectin 7 plays a role in cell-cell adhesion apoptosis regulation and immune response modulation. It plays a role in the cell's apoptosis machinery and interacts with the cytochrome c release pathway. This protein does not typically form a part of large complexes functioning individually in most biological processes. It can influence keratinocyte migration indicating involvement in wound healing processes.

Pathways

Galectin 7 participates in the apoptosis and cell adhesion pathways. It works alongside proteins such as Bcl-2 and Bax to regulate programmed cell death by promoting apoptosis. Additionally it interacts with matrix metalloproteinases influencing the remodelling of extracellular matrix a critical component of tissue repair and immune response.

Galectin 7 is associated with cancer and psoriasis. In cancer it can act as a pro-apoptotic factor and modulates tumor progression often in concert with p53 a well-known tumor suppressor protein. In psoriasis aberrant expression of Galectin 7 correlates with impaired epidermal function and inflammation suggesting it may alter the disease course by influencing local immune responses and keratinocyte behavior.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

See full target information LGALS7

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:506 PubMed40634299

2025

MA-METTL3-dependent nuclear PANC754/PSPC1/H3K4me1 repression complex regulate immune evasive LGALS7 signal to enhance immunotherapy against colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfeng Zhang,Guilian Cao,Feng Li,Siyu Tang,Chenxi Wu,Min Jiang,Jingxin Ye,Shaoqing Ju,Fei Qian,Weifeng Ding

Scientific reports 14:23173 PubMed39369030

2024

ANGPTL4 plays a paradoxical role in gastric cancer through the LGALS7 and Hedgehog pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xie,Yukun Li,Tian Zeng,Tingyu Fan,Hanguo Shan,Gangqing Shi,Wenchao Zhou,Juan Zou,Xiaoyong Lei

International journal of molecular medicine 47:677-687 PubMed33416139

2021

Ultrasound‑targeted microbubble destruction‑mediated Galectin‑7‑siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wei,Yan Zheng,Weilin Zhang,Jing Tan,Hong Zheng

Journal of the American Society of Nephrology : JASN 31:2263-2277 PubMed32646856

2020

Lipocalin-2 Exacerbates Lupus Nephritis by Promoting Th1 Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Chen,Wenchao Li,Zhuoya Zhang,Xiaojun Tang,Shufang Wu,Genhong Yao,Kang Li,Dandan Wang,Yuemei Xu,Ruihai Feng,Xiaoxiao Duan,Xiangshan Fan,Liwei Lu,WanJun Chen,Chaojun Li,Lingyun Sun

Chemico-biological interactions 315:108900 PubMed31738905

2019

Predictive early gene signature during mouse Bhas 42 cell transformation induced by synthetic amorphous silica nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Anaïs Kirsch,Hélène Dubois-Pot-Schneider,Caroline Fontana,Hervé Schohn,Laurent Gaté,Yves Guichard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Galectin 7 antibody [EPR19903]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Flow Cytometry (Intracellular) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunoprecipitation - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemistry (Frozen sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Western blot - Anti-Galectin 7 antibody [EPR19903] (AB206435)

Western blot - Anti-Galectin 7 antibody [EPR19903] (AB206435)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise