Anti-Galectin 7 antibody [EPR4287]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 7 antibody [EPR4287] (AB108623)

Immunocytochemistry analysis of HaCaT (Human skin keratinocyte) cells labeling Galectin 7 with Purified ab108623 at 1 : 1000 dilution (0.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 7 antibody [EPR4287] (AB108623)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Galectin 7 with purified ab108623 at 1/1500 dilution (0.35 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Galectin 7 antibody [EPR4287] (AB108623)

Intracellular Flow Cytometry analysis of HaCaT (Human skin keratinocyte) cells labeling Galectin 7 with Purified ab108623 at 1/50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488 ,ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• IP

Lab

Immunoprecipitation - Anti-Galectin 7 antibody [EPR4287] (AB108623)

Purified ab108623 at 1/50 dilution (2μg) immunoprecipitating Galectin 7 in HaCaT whole cell lysate.
Lane 1 (input) : HaCaT (Human skin keratinocyte) whole cell lysate 10μg
Lane 2 (+) : ab108623 + HaCaT whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108623 in HaCaT whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 15 kDa

All lanes:

Immunoprecipitation - Anti-Galectin 7 antibody [EPR4287] (ab108623)

Predicted band size: 15 kDa

false

• WB

Unknown

Western blot - Anti-Galectin 7 antibody [EPR4287] (AB108623)

All lanes:

Western blot - Anti-Galectin 7 antibody [EPR4287] (ab108623) at 1/1000 dilution

All lanes:

Human skin lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 15 kDa

false