AB42879

Anti-Galectin 8/Gal-8 antibody

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(3 Publications)

Rabbit Polyclonal Galectin 8/Gal-8 antibody. Suitable for WB, ICC/IF and reacts with Recombinant full length protein, Human samples. Cited in 3 publications.

View Alternative Names

Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, Po66-CBP, PCTA-1, LGALS8

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody (AB42879)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody (AB42879)

ICC/IF image of ab42879 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42879, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Project2962****

Western blot - Anti-Galectin 8/Gal-8 antibody (AB42879)

ab42879 recognizes the full length tagged recombinant Galectin 8/Gal-8 protein which has an expected molecular weight of 65.5 kDa.

All lanes:

Western blot - Anti-Galectin 8/Gal-8 antibody (ab42879) at 1 µg/mL

All lanes:

Galectin 8/Gal-8 Recombinant Protein at 0.1 µg

Secondary

All lanes:

Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

Predicted band size: 36 kDa

Observed band size: 70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 8 also known as Gal-8 or GAL is a member of the galectin protein family which binds β-galactosides. The protein has another name anti-gal and possesses a molecular mass of approximately 36 kDa. It exhibits broad expression in various tissues including the immune system bones and gastrointestinal tract. Galectin-8 functions mechanically by binding to glycoproteins and glycolipids which facilitates cell adhesion and regulation processes.

Biological function summary

Galectin-8 impacts numerous cellular functions through its involvement in immunomodulation and cell migration. Gal-8 promotes adhesion between cells and the extracellular matrix. It is not a part of a larger protein complex but interacts with multiple cell surface receptors guiding them toward or away from specific locations. Galectins including Galectin-8 influence immune cell responses by managing the apoptosis of T-cells and modifying cytokine production.

Pathways

Galectin-8 engages in signaling pathways related to cellular adhesion and immune system modulation. It participates in the integrin-mediated cell adhesion pathway and contributes to the receptor signaling pathway associated with cytokines. Within these pathways Galectin-8 interacts with other proteins such as integrins and T-cell receptors influencing their activation internalization and cell surface retention.

Galectin-8 plays roles in cancer progression and inflammatory conditions. It is connected to cancer as it regulates angiogenesis cell invasion and metastasis. Galectin-8 interacts with fibronectin and vascular endothelial growth factor (VEGF) within the context of cancer. In inflammatory diseases Gal-8 influences immune cell migration and inflammation through its action on cytokine receptors contributing to conditions like rheumatoid arthritis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed : 22246324, PubMed : 28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed : 22246324, PubMed : 28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed : 22246324, PubMed : 28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed : 22246324). Also required to restrict infection of Picornaviridae viruses (PubMed : 28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed : 21288902).

See full target information LGALS8

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6622 PubMed40681505

2025

The potassium channel K2.1 shapes the morphology and function of brain endothelial cells via actin network remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Lichtenberg,Laura Vinnenberg,Falk Steffen,Isabelle Plegge,Nicholas Hanuscheck,Vera Dobelmann,Joel Gruchot,Christina B Schroeter,Haribaskar Ramachandran,Beatrice Wasser,Derya Bachir,Christopher Nelke,Jonas Franz,Christoph Riethmüller,Stefan Tenzer,Ute Distler,Christina Francisca Vogelaar,Kristina Kusche-Vihrog,Boris V Skryabin,Timofey S Rozhdestvensky,Albrecht Schwab,Jean Krutmann,Andrea Rossi,Thomas Budde,Stefan Bittner,Sven G Meuth,Tobias Ruck

Cell reports 42:112037 PubMed36701233

2023

The selective autophagy adaptor p62/SQSTM1 forms phase condensates regulated by HSP27 that facilitate the clearance of damaged lysosomes via lysophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth R Gallagher,Erika L F Holzbaur

PLoS pathogens 18:e1010736 PubMed35857795

2022

TBK1 is part of a galectin 8 dependent membrane damage recognition complex and drives autophagy upon Adenovirus endosomal escape.

Applications

Unspecified application

Species

Unspecified reactive species

Noémie Pied,Coralie F Daussy,Zoé Denis,Jessica Ragues,Muriel Faure,Richard Iggo,Mario P Tschan,Benoit Roger,Fabienne Rayne,Harald Wodrich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Galectin 8/Gal-8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody (AB42879)

Western blot - Anti-Galectin 8/Gal-8 antibody (AB42879)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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