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AB69631

Anti-Galectin 8/Gal-8 antibody

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(4 Publications)

Rabbit Polyclonal Galectin 8/Gal-8 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, Po66-CBP, PCTA-1, LGALS8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody (AB69631)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody (AB69631)

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue using ab69631 at 1 : 50 dilution.Left image un-treated. Right image treated with immunizing peptide.

Western blot - Anti-Galectin 8/Gal-8 antibody (AB69631)
  • WB

Unknown

Western blot - Anti-Galectin 8/Gal-8 antibody (AB69631)

All lanes:

Western blot - Anti-Galectin 8/Gal-8 antibody (ab69631) at 1/500 dilution

Lane 1:

extracts of 3T3 cells at 5 µg

Lane 2:

extracts of 3T3 cells at 5 µg with immunising peptide

Predicted band size: 36 kDa

Observed band size: 36 kDa,60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 8 also known as Gal-8 or GAL is a member of the galectin protein family which binds β-galactosides. The protein has another name anti-gal and possesses a molecular mass of approximately 36 kDa. It exhibits broad expression in various tissues including the immune system bones and gastrointestinal tract. Galectin-8 functions mechanically by binding to glycoproteins and glycolipids which facilitates cell adhesion and regulation processes.
Biological function summary

Galectin-8 impacts numerous cellular functions through its involvement in immunomodulation and cell migration. Gal-8 promotes adhesion between cells and the extracellular matrix. It is not a part of a larger protein complex but interacts with multiple cell surface receptors guiding them toward or away from specific locations. Galectins including Galectin-8 influence immune cell responses by managing the apoptosis of T-cells and modifying cytokine production.

Pathways

Galectin-8 engages in signaling pathways related to cellular adhesion and immune system modulation. It participates in the integrin-mediated cell adhesion pathway and contributes to the receptor signaling pathway associated with cytokines. Within these pathways Galectin-8 interacts with other proteins such as integrins and T-cell receptors influencing their activation internalization and cell surface retention.

Galectin-8 plays roles in cancer progression and inflammatory conditions. It is connected to cancer as it regulates angiogenesis cell invasion and metastasis. Galectin-8 interacts with fibronectin and vascular endothelial growth factor (VEGF) within the context of cancer. In inflammatory diseases Gal-8 influences immune cell migration and inflammation through its action on cytokine receptors contributing to conditions like rheumatoid arthritis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed : 22246324, PubMed : 28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed : 22246324, PubMed : 28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed : 22246324, PubMed : 28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed : 22246324). Also required to restrict infection of Picornaviridae viruses (PubMed : 28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed : 21288902).
See full target information LGALS8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e30422 PubMed38737270

2024

Inhibition of sugar-binding activity of Galectins-8 by thiogalactoside (TDG) attenuates secondary brain damage and improves long-term prognosis following intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Song,Hongying Bai,Si Chen,Yuanyuan Xing,Jiyu Lou

PLoS pathogens 19:e1011088 PubMed37352334

2023

Deficiency in Galectin-3, -8, and -9 impairs immunity to chronic Mycobacterium tuberculosis infection but not acute infection with multiple intracellular pathogens.

Applications

Unspecified application

Species

Unspecified reactive species

Huntly M Morrison,Julia Craft,Rafael Rivera-Lugo,Jeffery R Johnson,Guillaume R Golovkine,Samantha L Bell,Claire E Dodd,Erik Van Dis,Wandy L Beatty,Shally R Margolis,Teresa Repasy,Isaac Shaker,Angus Y Lee,Russell E Vance,Sarah A Stanley,Robert O Watson,Nevan J Krogan,Daniel A Portnoy,Bennett H Penn,Jeffery S Cox

Cancer discovery 5:168-81 PubMed25421439

2014

Aberrant glycosylation promotes lung cancer metastasis through adhesion to galectins in the metastatic niche.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan E Reticker-Flynn,Sangeeta N Bhatia

Nature communications 3:1122 PubMed23047680

2012

A combinatorial extracellular matrix platform identifies cell-extracellular matrix interactions that correlate with metastasis.

Applications

WB, IHC

Species

Mouse, Mouse

Nathan E Reticker-Flynn,David F Braga Malta,Monte M Winslow,John M Lamar,Mary J Xu,Gregory H Underhill,Richard O Hynes,Tyler E Jacks,Sangeeta N Bhatia
View all publications
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Product promise

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