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AB320016

Anti-Galectin 8/Gal-8 antibody [EPR26780-52] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Galectin 8/Gal-8 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Galectin-8, Gal-8, LGALS-8, Lgals8

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Sandwich ELISA - Anti-Galectin 8/Gal-8 antibody [EPR26780-52] - BSA and Azide free (Detector) (AB320016)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Galectin 8/Gal-8 antibody [EPR26780-52] - BSA and Azide free (Detector) (AB320016)

Sandwich ELISA of ab320014 with the capture antibody ab320015 dilution at 2 ug/mL and detector antibody ab320016 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26780-52

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab320016 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Galectin 8/Gal-8. The recommended pair for sandwich ELISA is:

Capture: ab320015, Mouse Galectin 8/Gal-8 Capture Antibody (unconjugated)

Detector: ab320016, Mouse Galectin 8/Gal-8 Detector Antibody (unconjugated)

The reference range value is 39.06-5000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 8 also known as Gal-8 or GAL is a member of the galectin protein family which binds β-galactosides. The protein has another name anti-gal and possesses a molecular mass of approximately 36 kDa. It exhibits broad expression in various tissues including the immune system bones and gastrointestinal tract. Galectin-8 functions mechanically by binding to glycoproteins and glycolipids which facilitates cell adhesion and regulation processes.
Biological function summary

Galectin-8 impacts numerous cellular functions through its involvement in immunomodulation and cell migration. Gal-8 promotes adhesion between cells and the extracellular matrix. It is not a part of a larger protein complex but interacts with multiple cell surface receptors guiding them toward or away from specific locations. Galectins including Galectin-8 influence immune cell responses by managing the apoptosis of T-cells and modifying cytokine production.

Pathways

Galectin-8 engages in signaling pathways related to cellular adhesion and immune system modulation. It participates in the integrin-mediated cell adhesion pathway and contributes to the receptor signaling pathway associated with cytokines. Within these pathways Galectin-8 interacts with other proteins such as integrins and T-cell receptors influencing their activation internalization and cell surface retention.

Galectin-8 plays roles in cancer progression and inflammatory conditions. It is connected to cancer as it regulates angiogenesis cell invasion and metastasis. Galectin-8 interacts with fibronectin and vascular endothelial growth factor (VEGF) within the context of cancer. In inflammatory diseases Gal-8 influences immune cell migration and inflammation through its action on cytokine receptors contributing to conditions like rheumatoid arthritis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy. Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture. Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52. Required to restrict infection of bacterial invasion such as S.typhimurium. Also required to restrict infection of Picornaviridae viruses. Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans.
See full target information Lgals8
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Product promise

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For full details, please see our Terms & Conditions

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