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Rabbit Recombinant Monoclonal Galectin 8/Gal-8 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment samples. Cited in 15 publications.


Images

Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] (AB109519), expandable thumbnail
  • Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (AB109519), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] (AB109519), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (AB109519), expandable thumbnail
  • Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (AB109519), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Recombinant fragment
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Recombinant fragment
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species
Recombinant fragment
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed:22246324, PubMed:28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed:22246324, PubMed:28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed:22246324, PubMed:28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed:22246324). Also required to restrict infection of Picornaviridae viruses (PubMed:28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed:21288902).

Alternative names

Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, Po66-CBP, PCTA-1, LGALS8

Recommended products

Rabbit Recombinant Monoclonal Galectin 8/Gal-8 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment samples. Cited in 15 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4857
Purification technique
Affinity purification Protein A
Specificity

This antibody has weak cross-reactivity with Galectin 9.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Galectin 8 also known as Gal-8 or GAL is a member of the galectin protein family which binds β-galactosides. The protein has another name anti-gal and possesses a molecular mass of approximately 36 kDa. It exhibits broad expression in various tissues including the immune system bones and gastrointestinal tract. Galectin-8 functions mechanically by binding to glycoproteins and glycolipids which facilitates cell adhesion and regulation processes.

Biological function summary

Galectin-8 impacts numerous cellular functions through its involvement in immunomodulation and cell migration. Gal-8 promotes adhesion between cells and the extracellular matrix. It is not a part of a larger protein complex but interacts with multiple cell surface receptors guiding them toward or away from specific locations. Galectins including Galectin-8 influence immune cell responses by managing the apoptosis of T-cells and modifying cytokine production.

Pathways

Galectin-8 engages in signaling pathways related to cellular adhesion and immune system modulation. It participates in the integrin-mediated cell adhesion pathway and contributes to the receptor signaling pathway associated with cytokines. Within these pathways Galectin-8 interacts with other proteins such as integrins and T-cell receptors influencing their activation internalization and cell surface retention.

Associated diseases and disorders

Galectin-8 plays roles in cancer progression and inflammatory conditions. It is connected to cancer as it regulates angiogenesis cell invasion and metastasis. Galectin-8 interacts with fibronectin and vascular endothelial growth factor (VEGF) within the context of cancer. In inflammatory diseases Gal-8 influences immune cell migration and inflammation through its action on cytokine receptors contributing to conditions like rheumatoid arthritis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    Purified ab109519 at 1/30 dilution (2μg) immunoprecipitating Galectin 8/Gal-8 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): ab109519 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109519 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/10,000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 36/40 kDa

    All lanes: Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    Predicted band size: 36 kDa

  • Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    Blocking and diluting buffer 5% NFDM/TBST

    All lanes: Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/10000 dilution

    All lanes: LNCAP (human prostat carcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa

    Observed band size: 40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    ab109519 staining Galectin 8/Gal-8 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody ab109519 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
    Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)

  • Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    ab109519 staining Galectin 8/Gal-8 in the human cell line HEK293 (human embryonic kidney) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    All lanes: Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: LNCaP cell lysate at 10 µg

    Predicted band size: 36 kDa, 68 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    ab109519 has weak cross-reactivity with Galectin 9.

    Lanes 1 and 3: unpurified at 1/1000 dilution

    Lane 2: Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/200 dilution

    All lanes: Galectin 9 recombinant protein at 0.02 µg

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    ab109519 staining Galectin 8/Gal-8 in human prostatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    Unpurified ab109519, at a 1/100 dilution, staining Human prostatic adenocarcinoma, using Immunohistochemstry, Formalin/PFA-fixed paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    Overlay histogram showing HeLa cells stained with unpurified ab109519 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109519, 1/100) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519), expandable thumbnail

    Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519)

    All lanes: Western blot - Anti-Galectin 8/Gal-8 antibody [EPR4857] (ab109519) at 1/5000 dilution

    All lanes: Western blot - Recombinant Human Galectin 8/Gal-8 protein (Recombinant Human Galectin 8/Gal-8 protein ab99294) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Exposure time: 90s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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