Rabbit Recombinant Monoclonal Galectin 8/Gal-8 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment samples.
Images
![Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunoprecipitation-img4492.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983)")
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunocytochemistry-immunofluorescence-img37382.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983)")
![Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--flow-cytometry-intracellular-img32686.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68250.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83111.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (AB239983)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Recombinant fragment | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
8 products for Alternative Version
Target data
Function
Beta-galactoside-binding lectin that acts as a sensor of membrane damage caused by infection and restricts the proliferation of infecting pathogens by targeting them for autophagy (PubMed:22246324, PubMed:28077878). Detects membrane rupture by binding beta-galactoside ligands located on the lumenal side of the endosome membrane; these ligands becoming exposed to the cytoplasm following rupture (PubMed:22246324, PubMed:28077878). Restricts infection by initiating autophagy via interaction with CALCOCO2/NDP52 (PubMed:22246324, PubMed:28077878). Required to restrict infection of bacterial invasion such as S.typhimurium (PubMed:22246324). Also required to restrict infection of Picornaviridae viruses (PubMed:28077878). Has a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans (PubMed:21288902).
Alternative names
Galectin-8, Gal-8, Po66 carbohydrate-binding protein, Prostate carcinoma tumor antigen 1, Po66-CBP, PCTA-1, LGALS8
Recommended products
Rabbit Recombinant Monoclonal Galectin 8/Gal-8 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant fragment samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR4857
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has weak cross-reactivity with Galectin 9.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239983 is the carrier-free version of Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin 8 also known as Gal-8 or GAL is a member of the galectin protein family which binds β-galactosides. The protein has another name anti-gal and possesses a molecular mass of approximately 36 kDa. It exhibits broad expression in various tissues including the immune system bones and gastrointestinal tract. Galectin-8 functions mechanically by binding to glycoproteins and glycolipids which facilitates cell adhesion and regulation processes.
- Biological function summary
Galectin-8 impacts numerous cellular functions through its involvement in immunomodulation and cell migration. Gal-8 promotes adhesion between cells and the extracellular matrix. It is not a part of a larger protein complex but interacts with multiple cell surface receptors guiding them toward or away from specific locations. Galectins including Galectin-8 influence immune cell responses by managing the apoptosis of T-cells and modifying cytokine production.
- Pathways
Galectin-8 engages in signaling pathways related to cellular adhesion and immune system modulation. It participates in the integrin-mediated cell adhesion pathway and contributes to the receptor signaling pathway associated with cytokines. Within these pathways Galectin-8 interacts with other proteins such as integrins and T-cell receptors influencing their activation internalization and cell surface retention.
- Associated diseases and disorders
Galectin-8 plays roles in cancer progression and inflammatory conditions. It is connected to cancer as it regulates angiogenesis cell invasion and metastasis. Galectin-8 interacts with fibronectin and vascular endothelial growth factor (VEGF) within the context of cancer. In inflammatory diseases Gal-8 influences immune cell migration and inflammation through its action on cytokine receptors contributing to conditions like rheumatoid arthritis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunoprecipitation-img4492.jpg "Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)")
Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)
This data was developed using Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519, the same antibody clone in a different buffer formulation.
Purified Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 at 1/30 dilution (2μg) immunoprecipitating Galectin 8/Gal-8 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+): Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36/40 kDaAll lanes: Immunoprecipitation - Anti-Galectin 8/Gal-8 antibody [EPR4857] (Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519)
Predicted band size: 36 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunocytochemistry-immunofluorescence-img37382.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)")
Immunocytochemistry/ Immunofluorescence - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)
Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 staining Galectin 8/Gal-8 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519).
![Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--flow-cytometry-intracellular-img32686.jpg "Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)")
Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)
Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 staining Galectin 8/Gal-8 in the human cell line HEK293 (human embryonic kidney) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img68250.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)
Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 staining Galectin 8/Gal-8 in human prostatic carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img83111.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)
Unpurified Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519, at a 1/100 dilution, staining Human prostatic adenocarcinoma, using Immunohistochemstry, Formalin/PFA-fixed paraffin-embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--flow-cytometry-intracellular-img63147.jpg "Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)")
Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)
Overlay histogram showing HeLa cells stained with unpurified Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519, 1/100) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Galectin 8/Gal-8 antibody [EPR4857] ab109519).
![Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983), expandable thumbnail](https://content.abcam.com/products/images/galectin-8-gal-8-antibody-epr4857-bsa-and-azide-free-ab239983--flow-cytometry-intracellular-img63147.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Galectin 8/Gal-8 antibody [EPR4857] - BSA and Azide free (ab239983)")
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