Anti-galectin 9/Gal-9 antibody [1G3]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [1G3] (AB153673)

IHC image of galectin 9/Gal-9 staining in Human normal Tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab153673, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Lab

Western blot - Anti-galectin 9/Gal-9 antibody [1G3] (AB153673)

All lanes:

Western blot - Anti-galectin 9/Gal-9 antibody [1G3] (ab153673) at 5 µg/mL

Lane 1:

HeLa transfected with galectin 9 / Gal-9 whole cell lysate at 10 µg

Lane 2:

HeLa (wild type control) whole cell lysate at 10 µg

Secondary

All lanes:

Goat anti-mouse IgG polyclonal antibody (HRP conjugated) at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 18 kDa,35 kDa

true

Exposure time: 30s

• WB

CiteAb

Western blot - Anti-galectin 9/Gal-9 antibody [1G3] (AB153673)

galectin 9/Gal-9 western blot using anti-galectin 9/Gal-9 antibody [1G3] ab153673. Publication image and figure legend from Barjon, C., Niki, T., et al., 2012, Infect Agent Cancer, PubMed 22805533.

ab153673 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab153673 please see the product overview.

Western blot detection of galectin-9 isoforms using the 1G3 monoclonal antibody. A low amount of S and M galectin-9 isoforms are present in wild-type HeLa cells. These isoforms are readily detected following transfection with the appropriate expression plasmids. The L-isoform is expressed at a lower abundance than the two other forms. The three endogenous isoforms are detected in the protein extracts from the C15 and C666-1 NPC xenografts. The EBV-positive B-cell line REMB1 expresses a high amount of the M-isoform and a very low amount of L-isoform, however no S-isoform could be detected. In contrast with REMB1 cells, the BL2 cells derived from an EBV-negative B-cell lymphoma have no detectable galectin-9. The faint background bands visible after long exposure do not match with any galectin-9 isoform.

false