Anti-galectin 9/Gal-9 antibody (ab69630) is a rabbit polyclonal antibody detecting galectin 9/Gal-9 in Western Blot, IHC-P, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.
- Over 30 publications
- Trusted since 2008
Images
Publications
Filter by
- Molecular immunology 156:136-1472023Gal-9/Tim-3 signaling pathway activation suppresses the generation of Th17 cells and promotes the induction of Foxp3 regulatory T cells in renal ischemia-reperfusion injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYamei Wang,Ting Feng,Hong Li,Ying Xiong,Yuhong TaoPubMed 36921488
- iScience 25:1053292022Cancer cell intrinsic TIM-3 induces glioblastoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Guo,Shuai Shen,Gefei Guan,Chen Zhu,Cunyi Zou,Jingyuan Cao,Wen Cheng,Xiaoyan Xu,Juanhan Yu,Zhiguo Lin,Guoli Wang,Ling Chen,Peng Cheng,Anhua WuPubMed 36325060
- Cell death & disease 13:4382022Single-cell characterization of malignant phenotypes and microenvironment alteration in retinoblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Wu,Jiaqi Yang,Wei Xiao,Zehang Jiang,Shuxia Chen,Dianlei Guo,Ping Zhang,Chunqiao Liu,Huasheng Yang,Zhi XiePubMed 35523772
- Archives of gynecology and obstetrics 306:1211-12202022Galectin-8 and -9 as prognostic factors for cervical cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusanne Beyer,Maya Wehrmann,Sarah Meister,Theresa M Kolben,Fabian Trillsch,Alexander Burges,Bastian Czogalla,Elisa Schmoeckel,Sven Mahner,Udo Jeschke,Thomas KolbenPubMed 35377045
- Nature communications 13:11572022Obesity-induced galectin-9 is a therapeutic target in B-cell acute lymphoblastic leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiyoung Lee,Jamie A G Hamilton,Ganesh R Talekar,Anthony J Ross,Langston Michael,Manali Rupji,Bhakti Dwivedi,Sunil S Raikar,Jeremy Boss,Christopher D Scharer,Douglas K Graham,Deborah DeRyckere,Christopher C Porter,Curtis J HenryPubMed 35241678
- Journal of neurovirology 28:99-1122022HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKinga Grabowska,Katarzyna Macur,Sarah Zieschang,Lubaba Zaman,Nicole Haverland,Andrew Schissel,Brenda Morsey,Howard S Fox,Pawel CiborowskiPubMed 35175539
- International journal of immunopathology and pharmacology 35:205873842110610512021Splenic CD4 and CD8 T-cells highly expressed PD-1 and Tim-3 in cirrhotic patients with HCV infection and portal hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Huang,Rui Zhou,Haiyan Chen,Shu Zhang,Jun Li,Wei Wei,Jin Sun,Song Ren,Baohua Li,Hong Deng,Jun Yang,Fanpu Ji,Zongfang LiPubMed 34930041
- Oncoimmunology 10:20039562021BDNF expression in GISTs predicts poor prognosis when associated with PD-L1 positive tumor-infiltrating lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuang-Ang Tian,Wen-Ting Xu,Yue Sun,Jin Wang,Qing Ke,Meng-Jiao Yuan,Jin-Jin Wang,Chun Zhuang,Qian GongPubMed 34804639
- International immunopharmacology 100:1081552021Functional role of galectin-9 in directing human innate immune reactions to Gram-negative bacteria and T cell apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie Schlichtner,N Helge Meyer,Inna M Yasinska,Nijas Aliu,Steffen M Berger,Bernhard F Gibbs,Elizaveta Fasler-Kan,Vadim V SumbayevPubMed 34543981
- Journal of immunology (Baltimore, Md. : 1950) 206:2692-26992021Galectin-9 Targets NLRP3 for Autophagic Degradation to Limit Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Wang,Ying Qin,Hui Song,Lijuan Wang,Mutian Jia,Chunyuan Zhao,Mouchun Gong,Wei ZhaoPubMed 33963043
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LGALS9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link O00182
Consider this alternative
Reactivity data
Select an application| ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Expected | Expected | Expected | Tested |
| Rat | Expected | Predicted | Expected | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Binds galactosides (PubMed:18005988). Has high affinity for the Forssman pentasaccharide (PubMed:18005988). Ligand for HAVCR2/TIM3 (PubMed:16286920). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (PubMed:16286920). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth (By similarity). Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307). Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity). Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation (PubMed:23817958). Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection (PubMed:20209097). Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells (PubMed:16116184, PubMed:24465902). Inhibits degranulation and induces apoptosis of mast cells (PubMed:24465902). Induces maturation and migration of dendritic cells (PubMed:16116184, PubMed:25754930). Inhibits natural killer (NK) cell function (PubMed:23408620). Can transform NK cell phenotype from peripheral to decidual during pregnancy (PubMed:25578313). Astrocyte derived galectin-9 enhances microglial TNF production (By similarity). May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion (By similarity). Highly selective to the anion urate (By similarity). Isoform 2. Acts as an eosinophil chemoattractant (PubMed:9642261). It also inhibits angiogenesis (PubMed:24333696). Suppresses IFNG production by natural killer cells (By similarity).
Alternative names
Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, LGALS9
Recommended products
Anti-galectin 9/Gal-9 antibody (ab69630) is a rabbit polyclonal antibody detecting galectin 9/Gal-9 in Western Blot, IHC-P, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.
- Over 30 publications
- Trusted since 2008
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LGALS9 aa 50-100. The exact immunogen used to generate this antibody is proprietary information. Database link O00182
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-galectin 9/Gal-9 antibody (ab69630) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human, Mouse, Rat samples.
What is the molecular weight of galectin 9/Gal-9?
Anti-galectin 9/Gal-9 (ab69630) specifically detects a band for galectin 9/Gal-9 (UniProt: O00182) at a molecular weight of 40kDa.
Trusted by the scientific community
Anti-galectin 9/Gal-9 (ab69630) was first used in a scientific publication in 2008 and has been cited over 30 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-9 also known as Gal-9 or LGALS9 is a Β-galactoside-binding lectin with a molecular mass of around 40 kDa. It shows expression in various tissues including the thymus spleen and liver. The galectin-9 protein is involved in cell-cell and cell-matrix interactions where it binds to glycoproteins and glycolipids. Researchers commonly use methods like Galectin-9 ELISA to measure its levels in different biological samples.
- Biological function summary
Galectins like galectin-9 play essential roles in regulating diverse immune responses. Galectin-9 acts within the immune system by modulating inflammation and apoptosis. It acts as both a pro-apoptotic factor for T cells and a protein mediator in innate immune functions. Galectin-9 proteins can form complexes with other lectins and cell surface receptors contributing to cellular signaling processes.
- Pathways
Galectin-9 is integral to the immune system's functional networks. It participates in the T-cell regulation pathways and the innate immunity pathways. In these pathways it interacts with other proteins like TIM-3 which mediates T-cell apoptosis and contributes to immune tolerance. These interaction networks play important roles in maintaining the body's immune homeostasis.
- Associated diseases and disorders
Galectin-9 has associations with autoimmune diseases and cancer. Its regulatory effects on T cells connect it to autoimmune conditions where abnormal T-cell activation occurs. In cancer the alteration in galectin-9 expression levels affects tumor immunity. The interaction of galectin-9 with other proteins like TIM-3 is important in cancer progression making it a target of interest for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-galectin 9/Gal-9 antibody (ab69630)
ab69630 recognizes the partial tagged recombinant Human galectin 9 / Gal-9 protein (galectin 1 domain only) which has an expected molecular weight of 19 kDa.
All lanes: Western blot - Anti-galectin 9/Gal-9 antibody (ab69630) at 1/500 dilution
All lanes: Western blot - Recombinant Human galectin 9/Gal-9 protein (Recombinant Human galectin 9/Gal-9 protein ab93649) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Exposure time: 30s
Immunocytochemistry/ Immunofluorescence - Anti-galectin 9/Gal-9 antibody (ab69630)
Immunofluorescence analysis of NIH/3T3 cells using ab69630 at 1/500 dilution, with (right panel) or without (left panel) the immunising peptide.
Western blot - Anti-galectin 9/Gal-9 antibody (ab69630)
All lanes: Western blot - Anti-galectin 9/Gal-9 antibody (ab69630) at 1/500 dilution
Lane 1: Extracts from HepG2 cells at 5 µg
Lane 2: Extracts from HepG2 cells at 5 µg with immunising peptide
Predicted band size: 40 kDa
Observed band size: 40 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com