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Rabbit Recombinant Monoclonal galectin 9/Gal-9 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 6 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-galectin 9/Gal-9 antibody [EPR22214] (AB227046), expandable thumbnail
  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (AB227046), expandable thumbnail
  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (AB227046), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [EPR22214] (AB227046), expandable thumbnail
  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (AB227046), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)IHC-P
Human
Tested
Not recommended
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

5 products for Alternative Product

1 products for Alternative Version

Target data

Function

Binds galactosides (PubMed:18005988). Has high affinity for the Forssman pentasaccharide (PubMed:18005988). Ligand for HAVCR2/TIM3 (PubMed:16286920). Binding to HAVCR2 induces T-helper type 1 lymphocyte (Th1) death (PubMed:16286920). Also stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth (By similarity). Ligand for P4HB; the interaction retains P4HB at the cell surface of Th2 T-helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307). Ligand for CD44; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity). Promotes ability of mesenchymal stromal cells to suppress T-cell proliferation (PubMed:23817958). Expands regulatory T-cells and induces cytotoxic T-cell apoptosis following virus infection (PubMed:20209097). Activates ERK1/2 phosphorylation inducing cytokine (IL-6, IL-8, IL-12) and chemokine (CCL2) production in mast and dendritic cells (PubMed:24465902, PubMed:16116184). Inhibits degranulation and induces apoptosis of mast cells (PubMed:24465902). Induces maturation and migration of dendritic cells (PubMed:25754930, PubMed:16116184). Inhibits natural killer (NK) cell function (PubMed:23408620). Can transform NK cell phenotype from peripheral to decidual during pregnancy (PubMed:25578313). Astrocyte derived galectin-9 enhances microglial TNF production (By similarity). May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. May provide the molecular basis for urate flux across cell membranes, allowing urate that is formed during purine metabolism to efflux from cells and serving as an electrogenic transporter that plays an important role in renal and gastrointestinal urate excretion (By similarity). Highly selective to the anion urate (By similarity).Isoform 2Acts as an eosinophil chemoattractant (PubMed:9642261). It also inhibits angiogenesis (PubMed:24333696). Suppresses IFNG production by natural killer cells (By similarity).

Alternative names

Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, LGALS9

Recommended products

Rabbit Recombinant Monoclonal galectin 9/Gal-9 antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 6 publications.

Alternative names

Galectin-9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, LGALS9

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22214

Purification technique

Affinity purification Protein A

Specificity

This antibody is not recommended for mouse and rat in IHC.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Immunocytochemistry analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Cell line U937 (human histiocytic lymphoma cell line) cells labeling galectin 9/Gal-9 with ab227046 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
    The nuclear counterstain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).

    Confocal image showing nuclear and cytoplasmic staining in U937 cell line.

    Negative control: PANC-1.

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Lanes 1-4: Merged signal (red and green). Green - ab227046 observed at 34-39 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab227046 Anti-galectin 9/Gal-9 antibody [EPR22214] was shown to specifically react with galectin 9/Gal-9 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human Lgals9 (galectin 9/Gal-9) knockout A549 cell line ab266923 (knockout cell lysate Human Lgals9 (galectin 9/Gal-9) knockout A549 cell lysate ab256976) was used. Wild-type and galectin 9/Gal-9 knockout samples were subjected to SDS-PAGE. ab227046 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046) at 1/500 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: Galectin 9 knockout A549 cell lysate at 20 µg

    Lane 3: U-937 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 40 kDa

    Observed band size: 34-39 kDa

    This data was developed using the same antibody clone in a different buffer formulation (ab227046).

    Lanes 1-3: Merged signal (red and green). Green - ab227046 observed at 34-39 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab227046 Anti-galectin 9/Gal-9 antibody [EPR22214] was shown to specifically react with galectin in wild-type A549 cells. Loss of signal was observed when knockout sample Human Lgals9 (galectin 9/Gal-9) knockout A549 cell lysate ab256976 was used. Wild-type and galectin knockout samples were subjected to SDS-PAGE. ab227046 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Lanes 1 - 2: Merged signal (red and green). Green - ab227046 observed at 35 kDa. Red - loading control Mouse anti Histone H3 observed at 18 kDa.

    ab227046 was shown to react with galectin 9/Gal-9 in wild-type THP-1 cells in Western blot with loss of signal observed in LGALS9 knockout cell line Human Lgals9 (galectin 9/Gal-9) knockout THP-1 cell line ab269505 (knockout cell lysate Human Lgals9 (galectin 9/Gal-9) knockout THP-1 cell lysate ab269667). Wild-type THP-1 and LGALS9 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab227046 and Mouse anti Histone H3 overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046) at 1/1000 dilution

    Lane 1: Wild-type THP-1 cell lysate at 20 µg

    Lane 2: LGALS9 knockout THP-1 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 40 kDa

    Observed band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling galectin 9/Gal-9 with ab227046 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining on Kupffer cells of human liver (PMID: 20209097, PMID: 18202194) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression of the isoforms observed is consistent with what has been described in the literature (PMID 11886844, PMID: 22805533).

    Degraded fragments (13-15 kDa) have also been descried (PMID: 15811318).

    Negative control: PANC-1.

    All lanes: Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046) at 1/1000 dilution

    Lane 1: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

    Lane 2: HL-60 (human Acute Promyelocytic Leukemia promyeloblast), whole cell lysate at 20 µg

    Lane 3: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

    Lane 4: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa

    Observed band size: 13-15 kDa, 34-39 kDa

    Exposure time: 59s

  • Flow Cytometry (Intracellular) - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PANC-1 (human pancreatic epithelial cancinoma cell line) (Left) / U937 (human histiocytic lymphoma cell line) (Right) cell line labeling galectin 9/Gal-9 with ab227046 at 1/500 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    Negative Control - PANC-1 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling galectin 9/Gal-9 with ab227046 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear and cytoplasmic staining on epithelial cells of human colon (PMID: 18202194) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) cells labeling galectin 9/Gal-9 with ab227046 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing no staining in HEK-293T cells

    Negative control: HEK-293T (PMID: 11698107). The nuclear counterstain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    Secondary antibody only control: Used PBS instead of the primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046), expandable thumbnail

    Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression of the isoforms observed is consistent with what has been described in the literature (PMID 11886844, PMID: 22805533).

    Degraded fragments (13-15 kDa) has also been described (PMID: 15811318).

    Negative control: HEK-293T (PMID: 24333696).

    All lanes: Western blot - Anti-galectin 9/Gal-9 antibody [EPR22214] (ab227046) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg

    Lane 2: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa

    Observed band size: 13-15 kDa, 34-39 kDa

    Exposure time: 59s

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