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AB228533

Anti-GALK1 antibody

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(2 Publications)

Rabbit Polyclonal GALK1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GALK1.

View Alternative Names

GALK, GALK1, Galactokinase, Galactose kinase

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-GALK1 antibody (AB228533)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GALK1 antibody (AB228533)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GALK1 (green) using ab228533 at 1 : 500 dilution in ICC/IF. Cells were fixed in 100% methanol for 5 minutes.

Blue : Hoechst 33342 staining.

Western blot - Anti-GALK1 antibody (AB228533)
  • WB

Supplier Data

Western blot - Anti-GALK1 antibody (AB228533)

10% SDS-PAGE

All lanes:

Western blot - Anti-GALK1 antibody (ab228533) at 1/2000 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 42 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GALK1. The exact immunogen used to generate this antibody is proprietary information.

P51570

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALK1 also known as galactokinase 1 is an enzyme with a molecular mass of approximately 42 kDa. It catalyzes the phosphorylation of galactose to galactose-1-phosphate an important first step in galactose metabolism. GALK1 is expressed mainly in the liver kidney and brain where it plays a critical role in processing galactose derived from the diet. Its activity ensures the proper utilization of galactose for energy production and biosynthetic processes.
Biological function summary

The phosphorylation by GALK1 initiates the Leloir pathway facilitating efficient galactose metabolism. GALK1 does not form part of a larger protein complex but acts independently to enable the conversion of galactose to intermediates useful in glycolysis and glycogenesis. By doing so it supports normal cellular functions and contributes to the maintenance of cellular energy balance.

Pathways

GALK1 functions at the beginning of the Leloir pathway critical for galactose processing. This pathway includes the conversion of galactose-1-phosphate to glucose-1-phosphate a reaction controlled by related enzymes like GALT (galactose-1-phosphate uridylyltransferase). GALK1's activity is interconnected with glucose metabolism bridging galactose and glucose metabolic pathways ensuring galactose can be converted for energy production or storage.

Mutations in GALK1 can lead to galactokinase deficiency which causes galactosemia type II. This condition results in the accumulation of galactitol leading to cataracts development in early childhood. Galactokinase deficiency can also disturb normal carbohydrate metabolism indirectly affecting other proteins such as GALT. Understanding GALK1's function and genetic variants helps in diagnosing and managing galactosemia and its related complications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of a phosphate from ATP to alpha-D-galactose and participates in the first committed step in the catabolism of galactose.
See full target information GALK1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 10:280 PubMed40887473

2025

Focal adhesion kinase/Src family kinase axis-mediated tyrosine phosphorylation of metabolic enzymes facilitates tumor metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Jing Zhang,Yuheng Zhu,Yanmeng Zhu,Jingyuan Pang,Qingnan Wu,Yan Wang,Qimin Zhan

Cell death & disease 16:667 PubMed40890114

2025

MAP3K13-232aa encoded by circMAP3K13 enhances cisplatin-induced pyroptosis by directly binding to IKKα in gastric adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kaining Du,Xiaojing Zhang,Ying Qin,Huizhen Ma,Chuhan Bing,Shiqi Deng,Yang Chen,Jiequan Qin,Shanshan Chang,Siyu Xiao,Lehua Peng,Xiaoya Xie,Xianling Feng,Xianli Fu,Yanjie Wei,Xinmin Fan,Hassan Ashktorab,Duane Smoot,Zhe Jin,Yin Peng
View all publications
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Product promise

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