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AB153815

Anti-GALK2 antibody

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(1 Publication)

Rabbit Polyclonal GALK2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GALK2 aa 50 to C-terminus.

View Alternative Names

GK2, GALK2, N-acetylgalactosamine kinase, GalNAc kinase, Galactokinase 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALK2 antibody (AB153815)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALK2 antibody (AB153815)

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labeling GALK2 with ab153815 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GALK2 antibody (AB153815)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GALK2 antibody (AB153815)

Immunofluorescent analysis of methanol-fixed Hep3B cells labeling GALK2 with ab153815 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Western blot - Anti-GALK2 antibody (AB153815)
  • WB

Unknown

Western blot - Anti-GALK2 antibody (AB153815)

10 % SDS-PAGE

All lanes:

Western blot - Anti-GALK2 antibody (ab153815) at 1/5000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 50 kDa

false

Western blot - Anti-GALK2 antibody (AB153815)
  • WB

Unknown

Western blot - Anti-GALK2 antibody (AB153815)

10 % SDS-PAGE

All lanes:

Western blot - Anti-GALK2 antibody (ab153815) at 1/1000 dilution

All lanes:

Mouse kidney whole cell lysate at 50 µg

Predicted band size: 50 kDa,52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GALK2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01415

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein known as GALK2 also referred to as galactokinase 2 functions mechanically as a kinase that phosphorylates galactose and N-acetylgalactosamine. This enzyme is a part of the Leloir pathway of galactose metabolism. The GALK2 gene encodes the protein which has a molecular mass of approximately 50 kDa. Its expression primarily occurs in the cytoplasm with notable levels found in liver kidney and pancreas tissues. GALK2 is important for catalyzing the transfer of a phosphate group to the sugar substrates which is an important step in their metabolism.
Biological function summary

The protein plays a role in maintaining the homeostasis of galactose and N-acetylgalactosamine levels within the cell. As an enzyme involved in the metabolism of sugars GALK2 helps to convert galactose and its derivatives for energy production and biosynthetic pathways. Although GALK2 does not form large complexes it interacts with other enzymes in the Leloir pathway to facilitate efficient biochemical transformations. These interactions assist in regulating the flow and conversion of substrates through metabolic pathways.

Pathways

The involvement of GALK2 can be primarily ascribed to the galactose metabolic pathway which is essential for processing dietary galactose into glucose-1-phosphate. This pathway also includes enzymes such as galactose-1-phosphate uridylyltransferase (GALT) and UDP-galactose 4'-epimerase (GALE). Additionally GALK2 participates indirectly in the glycolytic pathway by converting galactose derivatives thereby linking carbohydrate metabolism to broader cellular processes like energy generation and cellular respiration.

Abnormalities in GALK2 function or expression have associations with disorders in galactose metabolism such as galactosemia. Galactosemia is a rare condition characterized by the inability to properly metabolize galactose often leading to harmful accumulation of galactose or its derivatives in the body. In addition to this improper GALK2 activity may also relate to certain liver disorders due to disruptions in metabolic processes. In the context of galactosemia GALK2's activity is significant as it connects with enzymes like GALT and GALE which also play roles in this disorder's metabolic pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts on GalNAc. Also acts as a galactokinase when galactose is present at high concentrations. May be involved in a salvage pathway for the reutilization of free GalNAc derived from the degradation of complex carbohydrates.
See full target information GALK2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:2020 PubMed29789585

2018

Hypoxia induces senescence of bone marrow mesenchymal stem cells via altered gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Junyue Xing,Yongquan Ying,Chenxi Mao,Yiwei Liu,Tingting Wang,Qian Zhao,Xiaoling Zhang,Fuxia Yan,Hao Zhang
View all publications
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Product promise

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