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AB231647

Anti-GALNS/Chondroitinase antibody

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(1 Publication)

Rabbit Polyclonal GALNS/Chondroitinase antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Recombinant full length protein - Rat, Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Rat N-acetylgalactosamine-6-sulfatase aa 150-400.

View Alternative Names

N-acetylgalactosamine-6-sulfatase, Chondroitinsulfatase, Galactose-6-sulfate sulfatase, N-acetylgalactosamine-6-sulfate sulfatase, Chondroitinase, GalNAc6S sulfatase, Galns

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNS/Chondroitinase antibody (AB231647)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNS/Chondroitinase antibody (AB231647)

Paraffin-embedded rat kidney tissue stained for GALNS/Chondroitinase using ab231647 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNS/Chondroitinase antibody (AB231647)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNS/Chondroitinase antibody (AB231647)

Paraffin-embedded rat kidney tissue stained for GALNS/Chondroitinase using ab231647 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-GALNS/Chondroitinase antibody (AB231647)
  • WB

Supplier Data

Western blot - Anti-GALNS/Chondroitinase antibody (AB231647)

All lanes:

Western blot - Anti-GALNS/Chondroitinase antibody (ab231647) at 3 µg/mL

Lane 1:

Mouse liver lysate

Lane 2:

Pig liver lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 56 kDa,58 kDa

false

Western blot - Anti-GALNS/Chondroitinase antibody (AB231647)
  • WB

Supplier Data

Western blot - Anti-GALNS/Chondroitinase antibody (AB231647)

All lanes:

Western blot - Anti-GALNS/Chondroitinase antibody (ab231647) at 3 µg/mL

All lanes:

Recombinant rat GALNS/Chondroitinase protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Rat N-acetylgalactosamine-6-sulfatase aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q32KJ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab231647 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chondroitinase also known as N-acetylgalactosamine-6-sulfatase (GALNS) is an enzyme involved in the degradation of glycosaminoglycans such as chondroitin sulfate and keratan sulfate. It is a lysosomal hydrolase enzyme that plays an important role in remodeling the extracellular matrix by cleaving sulfate groups from these molecules. The molecular mass of GALNS is approximately 60 kDa. GALNS expression primarily occurs in tissues like cartilage bones and connective tissues reflecting its function in skeletal development and maintenance.
Biological function summary

N-acetylgalactosamine-6-sulfatase acts as a critical player in catabolism breaking down complex polysaccharides in lysosomes. By degrading glycosaminoglycans it prevents accumulation of excessive polysaccharides in cells which might lead to cellular dysfunction. GALNS operates as a single molecule not forming any known complexes yet its activity is integral to maintaining cellular homeostasis and preventing pathological buildup of substrates within cells.

Pathways

GALNS functions in the glycosaminoglycan metabolism pathway essential for recycling cellular materials from the extracellular matrix. Another related pathway is the lysosomal degradation pathway where it relates to other enzymes like iduronate-2-sulfatase that also participate in the breakdown of complex molecules. These pathways collectively help in maintaining normal cellular function by facilitating the turnover of matrix components.

N-acetylgalactosamine-6-sulfatase relates to Morquio A syndrome a lysosomal storage disorder caused by deficiencies in GALNS activity. This leads to abnormal accumulation of keratan sulfate and chondroitin sulfate resulting in skeletal abnormalities and growth issues. GALNS has also connections to mucopolysaccharidosis IV where defects in this enzyme similarly cause buildup of glycosaminoglycans. Proteins like N-sulfoglucosamine sulfohydrolase show association with GALNS through shared pathways in these disorders highlighting the interconnected nature of enzyme functions in lysosomal storage diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information N-acetylgalactosamine-6-sulfatase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70098 PubMed39491498

2024

Fasudil Alleviates Postoperative Neurocognitive Disorders in Mice by Downregulating the Surface Expression of α5GABAAR in Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Jinpeng Dong,Zhun Wang,Lixuan Li,Mengxue Zhang,Sixuan Wang,Yuan Luo,Ying Dong,Xiaokun Wang,Yongan Wang,Kaiyuan Wang,Yiqing Yin
View all publications
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