Rabbit Polyclonal GALNT12 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GALNT12 aa 1-200.
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- Journal of translational medicine 20:5202022CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJungang Zhao,Rizhao Li,Jiacheng Li,Ziyan Chen,Zixia Lin,Baofu Zhang,Liming Deng,Gang Chen,Yi WangPubMed 36348351
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GALNT12 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXK2
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
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Target data
Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.
Alternative names
Polypeptide N-acetylgalactosaminyltransferase 12, Polypeptide GalNAc transferase 12, Protein-UDP acetylgalactosaminyltransferase 12, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 12, GalNAc-T12, pp-GaNTase 12, GALNT12
Recommended products
Rabbit Polyclonal GALNT12 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GALNT12 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GALNT12 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXK2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GALNT12 also known as polypeptide N-acetylgalactosaminyltransferase 12 is a member of the GALNT enzyme family. It has a molecular mass of approximately 64 kDa. This enzyme adds N-acetylgalactosamine to serine and threonine residues on proteins initiating O-glycosylation an essential post-translational modification. GALNT12 is expressed in various tissues with significant expression in the colon and small intestine. Its action is critical in producing mucin-type O-glycans which play a role in cellular signaling and protection.
- Biological function summary
Polypeptide N-acetylgalactosaminyltransferase 12 contributes to the glycosylation of proteins which modifies their function and stability. This enzyme is part of the family of N-acetylgalactosaminyltransferases which are involved in creating complex glycan structures. This modification aids in controlling cell-cell interactions modulating immune responses and stabilizing proteins. The activity of GALNT12 along with other family members ensures the diversity and specificity in glycan composition across different tissue types.
- Pathways
The enzyme GALNT12 functions within the metabolic pathway that is responsible for glycosphingolipid biosynthesis. This pathway plays a pivotal role in generating glycolipids essential for cellular membranes' function and structure. GALNT12's activity intersects with other glycosyltransferases such as GALNT1 and GALNT2 which work together to promote proper glycan assembly. These pathways contribute significantly to biological processes like cell adhesion and signal transduction.
- Associated diseases and disorders
GALNT12 has been linked to colorectal cancer and familial adenomatous polyposis. Mutations in GALNT12 can lead to abnormal glycosylation patterns affecting cell growth and apoptosis contributing to cancer development. Research has shown interactions with other proteins like APC and β-catenin highlighting its involvement in carcinogenesis. Understanding these interactions helps to explore potential therapeutic targets and diagnostic markers for colorectal tumors.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-GALNT12 antibody (ab101358)
12% SDS-PAGE
All lanes: Western blot - Anti-GALNT12 antibody (ab101358) at 1/1000 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 32 kDa
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