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AB101358

Anti-GALNT12 antibody

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(2 Publications)

Rabbit Polyclonal GALNT12 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GALNT12 aa 1-200.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 12, Polypeptide GalNAc transferase 12, Protein-UDP acetylgalactosaminyltransferase 12, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 12, GalNAc-T12, pp-GaNTase 12, GALNT12

1 Images
Western blot - Anti-GALNT12 antibody (AB101358)
  • WB

Unknown

Western blot - Anti-GALNT12 antibody (AB101358)

12% SDS-PAGE

All lanes:

Western blot - Anti-GALNT12 antibody (ab101358) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human GALNT12 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q8IXK2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT12 also known as polypeptide N-acetylgalactosaminyltransferase 12 is a member of the GALNT enzyme family. It has a molecular mass of approximately 64 kDa. This enzyme adds N-acetylgalactosamine to serine and threonine residues on proteins initiating O-glycosylation an essential post-translational modification. GALNT12 is expressed in various tissues with significant expression in the colon and small intestine. Its action is critical in producing mucin-type O-glycans which play a role in cellular signaling and protection.
Biological function summary

Polypeptide N-acetylgalactosaminyltransferase 12 contributes to the glycosylation of proteins which modifies their function and stability. This enzyme is part of the family of N-acetylgalactosaminyltransferases which are involved in creating complex glycan structures. This modification aids in controlling cell-cell interactions modulating immune responses and stabilizing proteins. The activity of GALNT12 along with other family members ensures the diversity and specificity in glycan composition across different tissue types.

Pathways

The enzyme GALNT12 functions within the metabolic pathway that is responsible for glycosphingolipid biosynthesis. This pathway plays a pivotal role in generating glycolipids essential for cellular membranes' function and structure. GALNT12's activity intersects with other glycosyltransferases such as GALNT1 and GALNT2 which work together to promote proper glycan assembly. These pathways contribute significantly to biological processes like cell adhesion and signal transduction.

GALNT12 has been linked to colorectal cancer and familial adenomatous polyposis. Mutations in GALNT12 can lead to abnormal glycosylation patterns affecting cell growth and apoptosis contributing to cancer development. Research has shown interactions with other proteins like APC and β-catenin highlighting its involvement in carcinogenesis. Understanding these interactions helps to explore potential therapeutic targets and diagnostic markers for colorectal tumors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.
See full target information GALNT12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 20:520 PubMed36348351

2022

CAFs-derived SCUBE1 promotes malignancy and stemness through the Shh/Gli1 pathway in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jungang Zhao,Rizhao Li,Jiacheng Li,Ziyan Chen,Zixia Lin,Baofu Zhang,Liming Deng,Gang Chen,Yi Wang

Cell death & disease 5:e1408 PubMed25188518

2014

miR455 is linked to hypoxia signaling and is deregulated in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

S Lalevée,O Lapaire,M Bühler
View all publications
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Product promise

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