JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB201196

Anti-GALNT12 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal GALNT12 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GALNT12 aa 50 to C-terminus.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 12, Polypeptide GalNAc transferase 12, Protein-UDP acetylgalactosaminyltransferase 12, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 12, GalNAc-T12, pp-GaNTase 12, GALNT12

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT12 antibody (AB201196)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT12 antibody (AB201196)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal stomach tissue labeling GALNT12 with ab201196 at 1/100 dilution.

Western blot - Anti-GALNT12 antibody (AB201196)
  • WB

Supplier Data

Western blot - Anti-GALNT12 antibody (AB201196)

All lanes:

Western blot - Anti-GALNT12 antibody (ab201196) at 1/500 dilution

All lanes:

A549 cell lysate

Predicted band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GALNT12 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IXK2

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT12 also known as polypeptide N-acetylgalactosaminyltransferase 12 is a member of the GALNT enzyme family. It has a molecular mass of approximately 64 kDa. This enzyme adds N-acetylgalactosamine to serine and threonine residues on proteins initiating O-glycosylation an essential post-translational modification. GALNT12 is expressed in various tissues with significant expression in the colon and small intestine. Its action is critical in producing mucin-type O-glycans which play a role in cellular signaling and protection.
Biological function summary

Polypeptide N-acetylgalactosaminyltransferase 12 contributes to the glycosylation of proteins which modifies their function and stability. This enzyme is part of the family of N-acetylgalactosaminyltransferases which are involved in creating complex glycan structures. This modification aids in controlling cell-cell interactions modulating immune responses and stabilizing proteins. The activity of GALNT12 along with other family members ensures the diversity and specificity in glycan composition across different tissue types.

Pathways

The enzyme GALNT12 functions within the metabolic pathway that is responsible for glycosphingolipid biosynthesis. This pathway plays a pivotal role in generating glycolipids essential for cellular membranes' function and structure. GALNT12's activity intersects with other glycosyltransferases such as GALNT1 and GALNT2 which work together to promote proper glycan assembly. These pathways contribute significantly to biological processes like cell adhesion and signal transduction.

GALNT12 has been linked to colorectal cancer and familial adenomatous polyposis. Mutations in GALNT12 can lead to abnormal glycosylation patterns affecting cell growth and apoptosis contributing to cancer development. Research has shown interactions with other proteins like APC and β-catenin highlighting its involvement in carcinogenesis. Understanding these interactions helps to explore potential therapeutic targets and diagnostic markers for colorectal tumors.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.
See full target information GALNT12
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:55 PubMed38218866

2024

Metabolic reprogramming based on RNA sequencing of gemcitabine-resistant cells reveals the FASN gene as a therapeutic for bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Zhou,Kaixuan Du,Yiheng Dai,Youmiao Zeng,Yongbo Luo,Mengda Ren,Wenbang Pan,Yuanhao Liu,Lailai Zhang,Ronghui Zhu,Dapeng Feng,Fengyan Tian,Chaohui Gu

Nature 615:168-174 PubMed36813961

2023

Microbiota-derived 3-IAA influences chemotherapy efficacy in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Tintelnot,Yang Xu,Till R Lesker,Martin Schönlein,Leonie Konczalla,Anastasios D Giannou,Penelope Pelczar,Dominik Kylies,Victor G Puelles,Agata A Bielecka,Manuela Peschka,Filippo Cortesi,Kristoffer Riecken,Maximilian Jung,Lena Amend,Tobias S Bröring,Marija Trajkovic-Arsic,Jens T Siveke,Thomas Renné,Danmei Zhang,Stefan Boeck,Till Strowig,Faik G Uzunoglu,Cenap Güngör,Alexander Stein,Jakob R Izbicki,Carsten Bokemeyer,Marianne Sinn,Alec C Kimmelman,Samuel Huber,Nicola Gagliani
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB101358

Anti-GALNT12 antibody

Western blot - Anti-GALNT12 antibody (AB101358)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB109489

Anti-Human IgG antibody [EPR4421]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

  • 4
  • 3
Primary Antibodies

AB172509

Anti-CIKS/ACT1 antibody

Western blot - Anti-CIKS/ACT1 antibody (AB172509)

  • 0
  • 0
Assay Kits

AB279412

Human Fatty Acid Synthase ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Fatty Acid Synthase ELISA Kit (AB279412)

  • 0
  • 0
Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com