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AB262868

Anti-GALNT2 antibody

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(3 Publications)

Rabbit Polyclonal GALNT2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 2 aa 400 to C-terminus.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, Protein-UDP acetylgalactosaminyltransferase 2, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, pp-GaNTase 2, GALNT2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT2 antibody (AB262868)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT2 antibody (AB262868)

Paraffin-embedded human pancreas tissue stained for GALNT2 using ab262868 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GALNT2 antibody (AB262868)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GALNT2 antibody (AB262868)

PFA-fixed, Triton X-100 permeabilized human U-2 OS cells stained for GALNT2 (green) using ab262868 at 4 μg/ml in ICC/IF.

Western blot - Anti-GALNT2 antibody (AB262868)
  • WB

Unknown

Western blot - Anti-GALNT2 antibody (AB262868)

Loading control : GAPDH.

All lanes:

Western blot - Anti-GALNT2 antibody (ab262868) at 0.4 µg/mL

Lane 1:

HeLa (transfected with control siRNA) cell lysate

Lanes 2 - 3:

HeLa (transfected with target-specific siRNA) cell lysate

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q10471

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GALNT2 protein also known as polypeptide N-acetylgalactosaminyltransferase 2 facilitates the initial step of mucin-type O-glycosylation by transferring N-acetylgalactosamine to serine or threonine residues on proteins. This enzyme has a molecular mass of approximately 69 kDa. GALNT2 expression occurs across various tissues with higher levels observed in the liver and small intestine. It plays a role in the modification of secreted and membrane-bound mucin glycoproteins.
Biological function summary

The function of GALNT2 extends beyond simple glycosylation. It contributes to protein sorting and stability within the Golgi apparatus. GALNT2 functions as a solitary entity and not within a larger protein complex. Its enzymatic activity has regulatory implications in lipid metabolism and signal transduction influencing the bioavailability of certain hormones and receptors by modifying glycans on their surface.

Pathways

GALNT2 plays roles in both the O-glycan biosynthesis pathway and the Wnt signaling pathway. In the O-glycan biosynthesis pathway GALNT2 interacts with other glycosyltransferases to ensure the proper glycosylation of proteins. Within the Wnt signaling pathway it indirectly influences the stability of certain proteins like frizzled receptors which are necessary for effective signaling. These pathways highlight GALNT2's integrative role in cellular processes such as development and homeostasis.

GALNT2 has an association with cardiovascular diseases and metabolic syndrome. Research shows that altered glycosylation patterns mediated by GALNT2 can affect the functionality of apolipoproteins like APOC3 thereby influencing lipid levels in the blood. Dysregulation of GALNT2 activity can lead to imbalances in these metabolic processes contributing to the pathogenesis of these conditions. Understanding GALNT2’s role presents potential therapeutic angles for managing such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. Involved in O-linked glycosylation of APOC-III, ANGPTL3 and PLTP. It participates in the regulation of HDL-C metabolism (PubMed : 27508872, PubMed : 32293671).
See full target information Polypeptide N-acetylgalactosaminyltransferase 2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2535 PubMed40087276

2025

ER O-glycosylation in synovial fibroblasts drives cartilage degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Le Son Tran,Joanne Chia,Xavier Le Guezennec,Keit Min Tham,Anh Tuan Nguyen,Virginie Sandrin,Way Cherng Chen,Tan Tong Leng,Sreedharan Sechachalam,Khai Pang Leong,Frederic A Bard

Nature chemical biology 21:193-202 PubMed38907113

2024

Orpinolide disrupts a leukemic dependency on cholesterol transport by inhibiting OSBP.

Applications

Unspecified application

Species

Unspecified reactive species

Marko Cigler,Hana Imrichova,Fabian Frommelt,Lucie Caramelle,Laura Depta,Andrea Rukavina,Chrysanthi Kagiou,J Thomas Hannich,Cristina Mayor-Ruiz,Giulio Superti-Furga,Sonja Sievers,Alison Forrester,Luca Laraia,Herbert Waldmann,Georg E Winter

Nature nanotechnology 18:657-666 PubMed36781994

2023

Machine-learning-assisted single-vessel analysis of nanoparticle permeability in tumour vasculatures.

Applications

Unspecified application

Species

Unspecified reactive species

Mingsheng Zhu,Jie Zhuang,Zhe Li,Qiqi Liu,Rongping Zhao,Zhanxia Gao,Adam C Midgley,Tianyi Qi,Jingwei Tian,Zhixuan Zhang,Deling Kong,Jie Tian,Xiyun Yan,Xinglu Huang
View all publications
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Product promise

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