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AB248920

Anti-GALNT2 antibody [EPR9605(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal GALNT2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 2, Polypeptide GalNAc transferase 2, Protein-UDP acetylgalactosaminyltransferase 2, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, pp-GaNTase 2, GALNT2

1 Images
Western blot - Anti-GALNT2 antibody [EPR9605(B)] - BSA and Azide free (AB248920)
  • WB

Unknown

Western blot - Anti-GALNT2 antibody [EPR9605(B)] - BSA and Azide free (AB248920)

This data was developed using ab140637, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GALNT2 antibody [EPR9605(B)] (<a href='/en-us/products/primary-antibodies/galnt2-antibody-epr9605b-ab140637'>ab140637</a>) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Lane 5:

Human fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9605(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248920 is the carrier-free version of ab140637.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The GALNT2 protein also known as polypeptide N-acetylgalactosaminyltransferase 2 facilitates the initial step of mucin-type O-glycosylation by transferring N-acetylgalactosamine to serine or threonine residues on proteins. This enzyme has a molecular mass of approximately 69 kDa. GALNT2 expression occurs across various tissues with higher levels observed in the liver and small intestine. It plays a role in the modification of secreted and membrane-bound mucin glycoproteins.
Biological function summary

The function of GALNT2 extends beyond simple glycosylation. It contributes to protein sorting and stability within the Golgi apparatus. GALNT2 functions as a solitary entity and not within a larger protein complex. Its enzymatic activity has regulatory implications in lipid metabolism and signal transduction influencing the bioavailability of certain hormones and receptors by modifying glycans on their surface.

Pathways

GALNT2 plays roles in both the O-glycan biosynthesis pathway and the Wnt signaling pathway. In the O-glycan biosynthesis pathway GALNT2 interacts with other glycosyltransferases to ensure the proper glycosylation of proteins. Within the Wnt signaling pathway it indirectly influences the stability of certain proteins like frizzled receptors which are necessary for effective signaling. These pathways highlight GALNT2's integrative role in cellular processes such as development and homeostasis.

GALNT2 has an association with cardiovascular diseases and metabolic syndrome. Research shows that altered glycosylation patterns mediated by GALNT2 can affect the functionality of apolipoproteins like APOC3 thereby influencing lipid levels in the blood. Dysregulation of GALNT2 activity can lead to imbalances in these metabolic processes contributing to the pathogenesis of these conditions. Understanding GALNT2’s role presents potential therapeutic angles for managing such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a broad spectrum of substrates for peptides such as EA2, Muc5AC, Muc1a, Muc1b. Probably involved in O-linked glycosylation of the immunoglobulin A1 (IgA1) hinge region. Involved in O-linked glycosylation of APOC-III, ANGPTL3 and PLTP. It participates in the regulation of HDL-C metabolism (PubMed : 27508872, PubMed : 32293671).
See full target information Polypeptide N-acetylgalactosaminyltransferase 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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