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AB151329

Anti-GALNT6 antibody

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(7 Publications)

Rabbit Polyclonal GALNT6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6 aa 1-550.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 6, Polypeptide GalNAc transferase 6, Protein-UDP acetylgalactosaminyltransferase 6, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6, pp-GaNTase 6, GALNT6

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-GALNT6 antibody (AB151329)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GALNT6 antibody (AB151329)

Immunofluorescent analysis of methanol-fixed A431 cells labeling GALNT6 with ab151329 at 1/200 dilution. Right panel co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT6 antibody (AB151329)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT6 antibody (AB151329)

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft labeling GALNT6 with ab151329 at 1/500 dilution.

Western blot - Anti-GALNT6 antibody (AB151329)
  • WB

Unknown

Western blot - Anti-GALNT6 antibody (AB151329)

7.5% SDS PAGE

All lanes:

Western blot - Anti-GALNT6 antibody (ab151329) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Western blot - Anti-GALNT6 antibody (AB151329)
  • WB

Supplier Data

Western blot - Anti-GALNT6 antibody (AB151329)

Samples were separated by 7.5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-GALNT6 antibody (ab151329) at 1/1000 dilution

Lane 1:

CaCo-2 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6 aa 1-550. The exact immunogen used to generate this antibody is proprietary information.

Q8NCL4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT6 also known as UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 6 is an enzyme weighing approximately 68 kDa. This enzyme belongs to the family of GalNAc transferases. It is known for its role in catalyzing the transfer of N-acetylgalactosamine to serine and threonine residues on proteins initiating O-linked glycosylation. GALNT6 is found expressed in various tissues but notably in the mammary gland and placenta.
Biological function summary

The enzyme GALNT6 plays a significant role in the modification of proteins through O-glycosylation impacting protein stability and function. It belongs to a larger group of glycosylation enzymes that participate in the modification and maturation of secreted and membrane-bound proteins. The function of GALNT6 influences the formation and activity of a variety of protein complexes although it itself does not form a complex. Through its action GALNT6 impacts cell signaling and adhesion processes.

Pathways

GALNT6 actively participates in the O-glycan biosynthesis pathway which is fundamental for generating proper glycoprotein structures. This enzyme works in conjunction with related enzymes like GALNT2 which also catalyze similar glycosylation reactions in the same pathway. Furthermore GALNT6 indirectly contributes to cellular pathways associated with cell growth and differentiation by altering the glycans on signaling molecules.

GALNT6 has been linked to certain types of cancer including breast cancer due to its role in glycoprotein formation that can affect cell adhesion and migration. Research also implicates GALNT6 in tumor progression potentially influencing the expression or function of proteins like mucins which are glycoproteins involved in cellular protection and signaling. Abnormal GALNT6 activity has further connections to lung cancer where its altered glycosylation patterns could modulate interactions with other enzymes and proteins important to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor (PubMed : 10464263, PubMed : 31932717). May participate in synthesis of oncofetal fibronectin (PubMed : 10464263). Has activity toward MUC1A, MUC2, EA2 and fibronectin peptides (PubMed : 10464263). Glycosylates FGF23 (PubMed : 31932717).
See full target information Polypeptide N-acetylgalactosaminyltransferase 6
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1332 PubMed40659945

2025

GALNT6 associated with O-GlcNAcylation contributes to the tumorigenesis of oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Yan,Xuan Tang,Yingying Zhou,Xin Xiong

Cancer cell international 24:308 PubMed39245709

2024

GALNT6 promotes bladder cancer malignancy and immune escape by epithelial-mesenchymal transition and CD8 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxin Sun,Haotian Wu,Ling Tang,Abdullah Al-Danakh,Yuli Jian,Li Gong,Congchen Li,Xiao Yu,Guang Zeng,Qiwei Chen,Deyong Yang,Shujing Wang

Archives of medical science : AMS 21:974-990 PubMed40741237

2024

Downregulation of expression inhibits malignant progression of breast cancer by targeting the pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Liu,Qiuli Du,Yong Yang

Frontiers in oncology 13:1097772 PubMed36925932

2023

Knocking down GALNT6 promotes pyroptosis of pancreatic ductal adenocarcinoma cells through NF-κB/NLRP3/GSDMD and GSDME signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyang Ding,Jingyu Liu,Honghui Lv,Yanlin Zhu,Yumiao Chen,Hui Peng,Sairong Fan,Xiaoming Chen

Journal of Cancer 12:7413-7421 PubMed35003361

2021

GALNT6 Knockdown Inhibits the Proliferation and Migration of Colorectal Cancer Cells and Increases the Sensitivity of Cancer Cells to 5-FU.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangdong Peng,Xueru Chen,Xiuting Zhu,Ling Chen

Cell death & disease 11:352 PubMed32393740

2020

GALNT6 promotes invasion and metastasis of human lung adenocarcinoma cells through O-glycosylating chaperone protein GRP78.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Song,Wenwen Liu,Jianzhen Wang,Junxia Hao,Yingyan Wang,Xin You,Xiaohui Du,Yang Zhou,Jing Ben,Xinri Zhang,Mingliang Ye,Qi Wang

International journal of molecular sciences 20: PubMed31071912

2019

The polypeptide GALNT6 Displays Redundant Functions upon Suppression of its Closest Homolog GALNT3 in Mediating Aberrant O-Glycosylation, Associated with Ovarian Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Razan Sheta,Magdalena Bachvarova,Elizabeth Macdonald,Stephane Gobeil,Barbara Vanderhyden,Dimcho Bachvarov
View all publications
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