Rabbit Polyclonal GALNT6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6 aa 1-550.
Images
Publications
Filter by
- Frontiers in oncology 13:10977722023Knocking down GALNT6 promotes pyroptosis of pancreatic ductal adenocarcinoma cells through NF-κB/NLRP3/GSDMD and GSDME signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengyang Ding,Jingyu Liu,Honghui Lv,Yanlin Zhu,Yumiao Chen,Hui Peng,Sairong Fan,Xiaoming ChenPubMed 36925932
- Journal of Cancer 12:7413-74212021GALNT6 Knockdown Inhibits the Proliferation and Migration of Colorectal Cancer Cells and Increases the Sensitivity of Cancer Cells to 5-FU.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangdong Peng,Xueru Chen,Xiuting Zhu,Ling ChenPubMed 35003361
- Cell death & disease 11:3522020GALNT6 promotes invasion and metastasis of human lung adenocarcinoma cells through O-glycosylating chaperone protein GRP78.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Song et. al.PubMed 32393740
- International journal of molecular sciences 20:2019The polypeptide GALNT6 Displays Redundant Functions upon Suppression of its Closest Homolog GALNT3 in Mediating Aberrant O-Glycosylation, Associated with Ovarian Cancer Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRazan Sheta et. al.PubMed 31071912
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6 aa 1-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NCL4
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor (PubMed:10464263, PubMed:31932717). May participate in synthesis of oncofetal fibronectin (PubMed:10464263). Has activity toward MUC1A, MUC2, EA2 and fibronectin peptides (PubMed:10464263). Glycosylates FGF23 (PubMed:31932717).
Alternative names
Polypeptide N-acetylgalactosaminyltransferase 6, Polypeptide GalNAc transferase 6, Protein-UDP acetylgalactosaminyltransferase 6, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6, pp-GaNTase 6, GALNT6
Recommended products
Rabbit Polyclonal GALNT6 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6 aa 1-550.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6 aa 1-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NCL4
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GALNT6 also known as UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 6 is an enzyme weighing approximately 68 kDa. This enzyme belongs to the family of GalNAc transferases. It is known for its role in catalyzing the transfer of N-acetylgalactosamine to serine and threonine residues on proteins initiating O-linked glycosylation. GALNT6 is found expressed in various tissues but notably in the mammary gland and placenta.
- Biological function summary
The enzyme GALNT6 plays a significant role in the modification of proteins through O-glycosylation impacting protein stability and function. It belongs to a larger group of glycosylation enzymes that participate in the modification and maturation of secreted and membrane-bound proteins. The function of GALNT6 influences the formation and activity of a variety of protein complexes although it itself does not form a complex. Through its action GALNT6 impacts cell signaling and adhesion processes.
- Pathways
GALNT6 actively participates in the O-glycan biosynthesis pathway which is fundamental for generating proper glycoprotein structures. This enzyme works in conjunction with related enzymes like GALNT2 which also catalyze similar glycosylation reactions in the same pathway. Furthermore GALNT6 indirectly contributes to cellular pathways associated with cell growth and differentiation by altering the glycans on signaling molecules.
- Associated diseases and disorders
GALNT6 has been linked to certain types of cancer including breast cancer due to its role in glycoprotein formation that can affect cell adhesion and migration. Research also implicates GALNT6 in tumor progression potentially influencing the expression or function of proteins like mucins which are glycoproteins involved in cellular protection and signaling. Abnormal GALNT6 activity has further connections to lung cancer where its altered glycosylation patterns could modulate interactions with other enzymes and proteins important to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT6 antibody (ab151329)
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft labeling GALNT6 with ab151329 at 1/500 dilution.
Western blot - Anti-GALNT6 antibody (ab151329)
7.5% SDS PAGE
All lanes: Western blot - Anti-GALNT6 antibody (ab151329) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 71 kDa
Immunocytochemistry/ Immunofluorescence - Anti-GALNT6 antibody (ab151329)
Immunofluorescent analysis of methanol-fixed A431 cells labeling GALNT6 with ab151329 at 1/200 dilution. Right panel co-stained with Hoechst 33342.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com