JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317215

Anti-GALNT6 antibody [HL2392]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal GALNT6 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 6, Polypeptide GalNAc transferase 6, Protein-UDP acetylgalactosaminyltransferase 6, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 6, GalNAc-T6, pp-GaNTase 6, GALNT6

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT6 antibody [HL2392] (AB317215)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT6 antibody [HL2392] (AB317215)

ab317215 detects GALNT6 protein at Golgi apparatus by immunohistochemical analysis. Sample : Paraffin-embedded mouse tissue. GALNT6 stained by ab317215 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)
  • WB

Supplier Data

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317215 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-GALNT6 antibody [HL2392] (ab317215) at 1/1000 dilution

Lane 1:

MCF-7 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)
  • WB

Supplier Data

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317215 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GALNT6 antibody [HL2392] (ab317215) at 1/1000 dilution

Lane 1:

Mouse stomach tissue extracts at 50 µg

Lane 2:

Mouse testis tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)
  • WB

Supplier Data

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)

Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE and the membrane was blotted with ab317215 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GALNT6 antibody [HL2392] (ab317215) at 1/1000 dilution

All lanes:

Rat stomach tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)
  • WB

Supplier Data

Western blot - Anti-GALNT6 antibody [HL2392] (AB317215)

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317215 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GALNT6 antibody [HL2392] (ab317215) at 1/1000 dilution

Lane 1:

Non-transfected (-) 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged GALNT3 transfected (+) 293T whole cell extracts at 30 µg

Lane 3:

DDDDK-tagged GALNT6 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2392

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Polypeptide N-acetylgalactosaminyltransferase 6.

Q8NCL4

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT6 also known as UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 6 is an enzyme weighing approximately 68 kDa. This enzyme belongs to the family of GalNAc transferases. It is known for its role in catalyzing the transfer of N-acetylgalactosamine to serine and threonine residues on proteins initiating O-linked glycosylation. GALNT6 is found expressed in various tissues but notably in the mammary gland and placenta.
Biological function summary

The enzyme GALNT6 plays a significant role in the modification of proteins through O-glycosylation impacting protein stability and function. It belongs to a larger group of glycosylation enzymes that participate in the modification and maturation of secreted and membrane-bound proteins. The function of GALNT6 influences the formation and activity of a variety of protein complexes although it itself does not form a complex. Through its action GALNT6 impacts cell signaling and adhesion processes.

Pathways

GALNT6 actively participates in the O-glycan biosynthesis pathway which is fundamental for generating proper glycoprotein structures. This enzyme works in conjunction with related enzymes like GALNT2 which also catalyze similar glycosylation reactions in the same pathway. Furthermore GALNT6 indirectly contributes to cellular pathways associated with cell growth and differentiation by altering the glycans on signaling molecules.

GALNT6 has been linked to certain types of cancer including breast cancer due to its role in glycoprotein formation that can affect cell adhesion and migration. Research also implicates GALNT6 in tumor progression potentially influencing the expression or function of proteins like mucins which are glycoproteins involved in cellular protection and signaling. Abnormal GALNT6 activity has further connections to lung cancer where its altered glycosylation patterns could modulate interactions with other enzymes and proteins important to disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor (PubMed : 10464263, PubMed : 31932717). May participate in synthesis of oncofetal fibronectin (PubMed : 10464263). Has activity toward MUC1A, MUC2, EA2 and fibronectin peptides (PubMed : 10464263). Glycosylates FGF23 (PubMed : 31932717).
See full target information Polypeptide N-acetylgalactosaminyltransferase 6
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com