AB254971

Anti-GALNT7 antibody

Name: Anti-GALNT7 antibody (ab254971) | Abcam
Brand: Abcam Limited
SKU: AB254971
Price: 655 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal GALNT7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GALNT7 aa 100-200.

View Alternative Names

N-acetylgalactosaminyltransferase 7, Polypeptide GalNAc transferase 7, Protein-UDP acetylgalactosaminyltransferase 7, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7, GalNAc-T7, pp-GaNTase 7, GALNT7

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Immunohistochemical analysis of human prostate tissue labeling GALNT7 with ab254971 at a 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Immunohistochemical analysis of human stomach tissue labeling GALNT7 with ab254971 at a 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Immunohistochemical analysis of human kidney tissue labeling GALNT7 with ab254971 at a 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GALNT7 antibody (AB254971)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GALNT7 antibody (AB254971)

PFA fixed, Triton X-100 permeabilized HaCaT (Human keratinocyte cell line) cells labeling GALNT7 using ab254971 at 4µg/ml (green) in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Immunohistochemical analysis of human liver tissue labeling GALNT7 with ab254971 at a 1/1000 dilution.

Supplier Data

Western blot - Anti-GALNT7 antibody (AB254971)

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Western blot - Anti-GALNT7 antibody (ab254971)

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Western blot - Anti-GALNT7 antibody (ab254971) at 0.4 µg/mL

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DNA Ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 75 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GALNT7 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q86SF2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT7 also known as polypeptide N-acetylgalactosaminyltransferase 7 is an enzyme involved in the initial step of O-glycosylation. This process involves the transfer of N-acetylgalactosamine (GalNAc) to serine or threonine residues on proteins. GALNT7 weighs approximately 72 kilodaltons and belongs to the family of GalNAc-transferases. The expression of GALNT7 is widespread predominantly found in the liver and pancreas but is also present in other tissues.

Biological function summary

GALNT7 catalyzes the addition of GalNAc to proteins in the Golgi apparatus. This modification is important for mucin-type O-glycan biosynthesis which influences protein folding stability and cellular signaling. GALNT7 does not function as part of a large protein complex but individually modifies substrate proteins. Its enzymatic activity impacts multiple cellular processes including cell adhesion and immune response.

Pathways

GALNT7 participates significantly in the mucin-type O-glycan biosynthesis pathway. This pathway is essential for generating glycoproteins commonly seen on cell surfaces and in secretions. GALNT7 does not act alone; it works alongside other GalNAc-transferases like GALNT2 which can influence substrate specificity and tissue-specific glycosylation. Another related pathway is the protein O-linked glycosylation pathway where GALNT7 interacts with proteins like MUC1 playing a role in glycoprotein configuration.

GALNT7 has associations with cancers particularly colorectal cancer. Abnormal glycosylation patterns resulting from altered GALNT7 activity can contribute to tumor growth and metastasis. Researchers study GALNT7 expression in cancer tissues to understand its role in oncogenesis. Additionally changes in GALNT7 activity may relate to inflammatory disorders such as Crohn's disease where mucin glycosylation influences intestinal barrier function and inflammation. The interaction between GALNT7 and MUC1 in these contexts is of significant interest especially in examining how it influences disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.

See full target information GALNT7

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

CytoJournal 22:5 PubMed39958879

2025

Relationship between transmembrane emp24 domain containing 2 expression and tumor stem cell characteristics in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhui Wu,Shunchu Zhang,Guimei Zou

Journal of Cancer 13:752-763 PubMed35154444

2022

Long non-coding RNA CCDC144NL-AS1 promotes cell proliferation by regulating the miR-363-3p/GALNT7 axis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Chaofan Peng,Jie Li,Dongsheng Zhang,Chuan Zhang,Kangpeng Jin,Dongjian Ji,Wen Peng,Junwei Tang,Yifei Feng,Yueming Sun

Nucleic acids research 49:9424-9443 PubMed34365507

2021

SDE2 is an essential gene required for ribosome biogenesis and the regulation of alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Jess Floro,Anqi Dai,Abigail Metzger,Alexandra Mora-Martin,Neil J Ganem,Daniel Cifuentes,Ching-Shyi Wu,Jasbir Dalal,Shawn M Lyons,Adam Labadorf,Rachel L Flynn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GALNT7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Immunocytochemistry/ Immunofluorescence - Anti-GALNT7 antibody (AB254971)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT7 antibody (AB254971)

Western blot - Anti-GALNT7 antibody (AB254971)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise