AB97645

Anti-GALNT7 antibody

Name: Anti-GALNT7 antibody (ab97645) | Abcam
Brand: Abcam Limited
SKU: AB97645

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(4 Publications)

Rabbit Polyclonal GALNT7 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GALNT7 aa 1-500.

View Alternative Names

N-acetylgalactosaminyltransferase 7, Polypeptide GalNAc transferase 7, Protein-UDP acetylgalactosaminyltransferase 7, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 7, GalNAc-T7, pp-GaNTase 7, GALNT7

1 Images

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Western blot - Anti-GALNT7 antibody (AB97645)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-GALNT7 antibody (ab97645) at 1/10000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 75 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human GALNT7 aa 1-500. The exact immunogen used to generate this antibody is proprietary information.

Q86SF2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT7 also known as polypeptide N-acetylgalactosaminyltransferase 7 is an enzyme involved in the initial step of O-glycosylation. This process involves the transfer of N-acetylgalactosamine (GalNAc) to serine or threonine residues on proteins. GALNT7 weighs approximately 72 kilodaltons and belongs to the family of GalNAc-transferases. The expression of GALNT7 is widespread predominantly found in the liver and pancreas but is also present in other tissues.

Biological function summary

GALNT7 catalyzes the addition of GalNAc to proteins in the Golgi apparatus. This modification is important for mucin-type O-glycan biosynthesis which influences protein folding stability and cellular signaling. GALNT7 does not function as part of a large protein complex but individually modifies substrate proteins. Its enzymatic activity impacts multiple cellular processes including cell adhesion and immune response.

Pathways

GALNT7 participates significantly in the mucin-type O-glycan biosynthesis pathway. This pathway is essential for generating glycoproteins commonly seen on cell surfaces and in secretions. GALNT7 does not act alone; it works alongside other GalNAc-transferases like GALNT2 which can influence substrate specificity and tissue-specific glycosylation. Another related pathway is the protein O-linked glycosylation pathway where GALNT7 interacts with proteins like MUC1 playing a role in glycoprotein configuration.

GALNT7 has associations with cancers particularly colorectal cancer. Abnormal glycosylation patterns resulting from altered GALNT7 activity can contribute to tumor growth and metastasis. Researchers study GALNT7 expression in cancer tissues to understand its role in oncogenesis. Additionally changes in GALNT7 activity may relate to inflammatory disorders such as Crohn's disease where mucin glycosylation influences intestinal barrier function and inflammation. The interaction between GALNT7 and MUC1 in these contexts is of significant interest especially in examining how it influences disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.

See full target information GALNT7

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology research 2024:5562293 PubMed39493373

2024

Exercise Attenuates Doxorubicin-Induced Myocardial Injury by Inhibiting TSHR and Regulating Macrophage Polarization Through miR-30d-5p/GALNT7.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Wu,Ruoyu Zhou,Hanxin Kong,Jieqiong Yang,Suijuan Liu,Xiaolin Wei,Kunzhi Li,Yunmei Zhang

Cancer cell international 20:117 PubMed32308562

2020

miR-125a-5p post-transcriptionally suppresses GALNT7 to inhibit proliferation and invasion in cervical cancer cells via the EGFR/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qinxue Cao,Ning Wang,Lu Ren,Jun Tian,Shaoqin Yang,Hailing Cheng

Developmental cell 50:57-72.e6 PubMed31105009

2019

Quantitative Super-Resolution Microscopy of the Mammalian Glycocalyx.

Applications

Unspecified application

Species

Unspecified reactive species

Leonhard Möckl,Kayvon Pedram,Anish R Roy,Venkatesh Krishnan,Anna-Karin Gustavsson,Oliver Dorigo,Carolyn R Bertozzi,W E Moerner

Molecular medicine reports 13:4085-94 PubMed27035333

2016

miR‑30a‑5p in the tumorigenesis of renal cell carcinoma: A tumor suppressive microRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Li,Yuchi Li,Duqun Chen,Lu Jin,Zhengming Su,Jiaju Liu,Hongfang Duan,Xiaoqing Li,Zhengyu Qi,Min Shi,Liangchao Ni,Shangqi Yang,Yaoting Gui,Xiangming Mao,Yun Chen,Yongqing Lai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GALNT7 antibody

Western blot - Anti-GALNT7 antibody (AB97645)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise