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AB121374

Anti-GALNT8 antibody

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(2 Publications)

Rabbit Polyclonal GALNT8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GALNT8 aa 500 to C-terminus.

View Alternative Names

Probable polypeptide N-acetylgalactosaminyltransferase 8, Polypeptide GalNAc transferase 8, Protein-UDP acetylgalactosaminyltransferase 8, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 8, GalNAc-T8, pp-GaNTase 8, GALNT8

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)

Immunohistochemical analysis of human testis tissue labeling GALNT8 with ab121374 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-GALNT8 antibody (AB121374)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GALNT8 antibody (AB121374)

Immunofluorescent staining of human cell line U-2 OS shows positivity in vesicles. Antibody staining is shown in green.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)

Immunohistochemical analysis of human cerebellum tissue labeling GALNT8 with ab121374 at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)

Immunohistochemical analysis of human liver tissue labeling GALNT8 with ab121374 at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNT8 antibody (AB121374)

Immunohistochemical analysis of human rectum tissue labeling GALNT8 with ab121374 at 1/1000 dilution.

Western blot - Anti-GALNT8 antibody (AB121374)
  • WB

Unknown

Western blot - Anti-GALNT8 antibody (AB121374)

All lanes:

Western blot - Anti-GALNT8 antibody (ab121374) at 1/250 dilution

Lane 1:

RT4 lysate

Lane 2:

U251 MG lysate

Predicted band size: 73 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GALNT8 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NY28

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT8 also known as polypeptide N-acetylgalactosaminyltransferase 8 is an enzyme that participates in the initial glycosylation steps transferring N-acetylgalactosamine to serine and threonine residues on proteins. This enzyme has a molecular mass of approximately 70 kDa. It is expressed in various tissues including the brain and liver. The enzyme belongs to the family of GALNTs which are key players in O-glycosylation a common form of post-translational modification.
Biological function summary

GALNT8 contributes to the synthesis of mucin-type O-glycans which are essential for the formation of mucous barriers and modulation of protein functions. The enzyme does not usually form part of large protein complexes but it can act in a sequential manner with other glycosyltransferases. This function is needed for proper protein stability and activity and for protecting proteins from proteolysis.

Pathways

GALNT8 participates in the O-glycan biosynthesis pathway. This pathway involves enzymes like GALNT2 and GALNT3 that help add sugar moieties to protein substrates. Additionally GALNT8 influences cell signaling and cellular interactions because glycosylation changes protein interactions and signaling receptor functions.

GALNT8 has links to conditions such as cancer and neurological disorders. Alterations in glycosylation patterns driven by GALNT8 affect tumor cell behavior including adhesion invasion and metastasis. In cancer GALNT8 may interact with proteins like MUC1 a mucin protein often overexpressed in tumors. Aberrant expression of GALNT8 may also influence neurological diseases where glycosylation changes impact neuronal communication and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.
See full target information GALNT8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology research and practice 2021:5960821 PubMed34239555

2021

lncRNA GAU1 Induces GALNT8 Overexpression and Potentiates Colorectal Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Tang,Haoyu Ruan,Liu Dong,Sihan Li,Zhiyuan Wu,Ming Guan

PloS one 12:e0186333 PubMed29040332

2017

The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour.

Applications

Unspecified application

Species

Unspecified reactive species

Ryuji Fukuzawa,Matthew R Anaka,Ian M Morison,Anthony E Reeve
View all publications
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Product promise

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