AB174275

Anti-GALNTL2 antibody [EPR11580]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal GALNTL2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

GALNTL2, UNQ770/PRO1564, GALNT15, Polypeptide N-acetylgalactosaminyltransferase 15, Polypeptide GalNAc transferase-like protein 2, Polypeptide N-acetylgalactosaminyltransferase-like protein 2, Protein-UDP acetylgalactosaminyltransferase-like protein 2, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 2, GalNAc-T-like protein 2, pp-GaNTase-like protein 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNTL2 antibody [EPR11580] (AB174275)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNTL2 antibody [EPR11580] (AB174275)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GALNTL2 with ab174275 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-GALNTL2 antibody [EPR11580] (AB174275)

All lanes:

Western blot - Anti-GALNTL2 antibody [EPR11580] (ab174275) at 1/1000 dilution

Lane 1:

K562 lysates at 10 µg

Lane 2:

Human placenta lysates at 10 µg

Lane 3:

Caco 2 lysates at 10 µg

Lane 4:

JAR lysates at 10 µg

Lane 5:

MCF7 lysates at 10 µg

Lane 6:

Human fetal brain lysates at 10 µg

Predicted band size: 73 kDa

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Lab

Western blot - Anti-GALNTL2 antibody [EPR11580] (AB174275)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-GALNTL2 antibody [EPR11580] (ab174275) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Exposure time: 3min

Carrier free

Anti-GALNTL2 antibody [EPR11580] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11580

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNTL2 also known as Polypeptide N-Acetylgalactosaminyltransferase-like 2 is an enzyme that belongs to the polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family. This enzyme has a molecular mass of approximately 63 kDa. It plays an important mechanical role in the initiation of mucin-type O-glycosylation by transferring N-acetylgalactosamine to the hydroxyl group of serine or threonine residues on target proteins. These modifications are essential for protein function. GALNTL2 is widely expressed in different human tissues including the liver brain and heart indicating its involvement in various physiological processes.

Biological function summary

The enzyme acts by modifying proteins to mediate their stability signaling and interaction with other molecules. GALNTL2 functions within a complex network of enzymes that manage glycosylation processes contributing to the modification of a variety of substrates. By altering protein structures through glycosylation it affects cell signaling pathways and immune responses. The enzyme's ability to modulate proteins influences processes like cellular adhesion and communication which are essential for maintaining tissue integrity and function.

Pathways

GALNTL2 plays a role in the O-glycosylation pathway which is important for proper protein folding and function. This pathway intersects with the protein processing pathway in the endoplasmic reticulum. GALNTL2 acts in conjunction with other members of the GalNAc-T family including GALNT1 and GALNT2 to fine-tune the glycosylation of proteins that pass through the secretory pathway. These processes are important for orchestrating responses to cellular stress and maintaining cellular homeostasis.

GALNTL2's dysfunction can relate to certain cancers and metabolic disorders. Abnormal glycosylation patterns due to mutations or misregulations in GALNTL2 have associations with cancer progression particularly in pathways involving cell-cell communication and adhesion influencing tumor growth and metastasis. Additionally metabolic disorders such as diabetes might also involve GALNTL2 through its interactions with signaling molecules like insulin. These associations highlight the importance of studying GALNTL2 for therapeutic targets in disease treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Although it displays a much weaker activity toward all substrates tested compared to GALNT2, it is able to transfer up to seven GalNAc residues to the Muc5AC peptide, suggesting that it can fill vicinal Thr/Ser residues in cooperation with other GALNT proteins. Prefers Muc1a as substrate.

See full target information GALNT15

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 12:1529495 PubMed40421300

2025

Proteomic insights into COPD pathogenesis and therapeutic targets: a causal analysis of circulating proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Min Luo,Ying Tang,Jing Qiu,Kai Yang,WenJing Dai,EnRong Fang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GALNTL2 antibody [EPR11580]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALNTL2 antibody [EPR11580] (AB174275)

Western blot - Anti-GALNTL2 antibody [EPR11580] (AB174275)

Western blot - Anti-GALNTL2 antibody [EPR11580] (AB174275)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise