Anti-GALR2 antibody [EPR24328A-120] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GALR2 antibody [EPR24328A-120] - BSA and Azide free (AB282821)

This data was developed using ab271019, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat dorsal root ganglia staining GALR2 with ab271019 at a 1/2000 dilution and ab177487 anti-NeuN used at 1/300 dilution.

Panel A : merged staining of anti-GALR2 (green; Opal™520) and anti-NeuN (magenta; Opal™570) on Rat dorsal root ganglia.

Panel B : anti-GALR2 stained on cytoplasm of neurons.

Panel C : anti-NeuN stained on nucleus and cytoplasm of neurons.

Panel D : Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in two rounds of staining : in the order of ab271019 and ab177487 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GALR2 antibody [EPR24328A-120] - BSA and Azide free (AB282821)

This data was developed using ab271019, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebral cortex tissue labeling GALR2 with ab271019 at 1/100 (5.17 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat cerebral cortex is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALR2 antibody [EPR24328A-120] - BSA and Azide free (AB282821)

This data was developed using ab271019, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat dorsal root ganglia tissue labelling GALR2 with ab271019 at 1/2000 (0.259 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining in rat dorsal root ganglia. The section was incubated with ab271019 at 4^oC overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GALR2 antibody [EPR24328A-120] - BSA and Azide free (AB282821)

This data was developed using ab271019, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labeling GALR2 with ab271019 at 1/100 (5.17 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (Green). Cytoplasmic staining on rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GALR2 antibody [EPR24328A-120] - BSA and Azide free (AB282821)

This data was developed using ab271019, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling GALR2 with ab271019 at 1/2000 (0.259 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining in rat cerebrum. The section was incubated with ab271019 at 4^oC overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GALR2 antibody [EPR24328A-120] - BSA and Azide free (AB282821)

This data was developed using ab271019, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling GALR2 with ab271019 at 1/100 (5.17 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green) Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.