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Rabbit Recombinant Monoclonal GALT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications.

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Images

Western blot - Anti-GALT antibody [EPR12555] (AB178406), expandable thumbnail
  • Western blot - Anti-GALT antibody [EPR12555] (AB178406), expandable thumbnail
  • Immunoprecipitation - Anti-GALT antibody [EPR12555] (AB178406), expandable thumbnail
  • Western blot - Anti-GALT antibody [EPR12555] (AB178406), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Expected
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays an important role in galactose metabolism.

Alternative names

Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase, GALT

Recommended products

Rabbit Recombinant Monoclonal GALT antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12555
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GALT also known as galactose-1-phosphate uridyltransferase is an enzyme involved in the Leloir pathway of galactose metabolism. The GALT protein facilitates the conversion of galactose-1-phosphate (Gal-1-P) and UDP-glucose to UDP-galactose and glucose-1-phosphate important steps in the cellular utilization of galactose. This enzyme has a molecular mass of approximately 43 kDa. GALT is expressed in various tissues and is notably present in the liver and erythrocytes where efficient galactose metabolism is essential.

Biological function summary

GALT plays a role in carbohydrate metabolism by regulating galactose conversion to glucose a principal energy source. It does not form part of a complex but works alongside other enzymes such as galactokinase and UDP-galactose 4'-epimerase in the Leloir pathway. This enzymatic action ensures that galactose from food sources is metabolized properly contributing to overall energy homeostasis within the body.

Pathways

GALT participates in the Leloir pathway which is essential for the breakdown and utilization of galactose. Through this pathway GALT interacts indirectly with related proteins like galactokinase and UDP-galactose 4'-epimerase. This enzymatic pathway is linked to complex carbohydrate metabolism and feeds into broader glucose metabolism processes underlining its importance in maintaining glucose levels.

Associated diseases and disorders

GALT is linked to classic galactosemia a rare genetic metabolic disorder. This condition arises from an impairment in the GALT enzyme leading to toxic accumulation of galactose-1-phosphate in the body. Individuals with classic galactosemia often experience symptoms like jaundice liver enlargement and can develop cataracts or mental developmental issues if not treated early. In the context of this disorder proteins such as galactokinase also play a role since they are upstream in the metabolic pathway that leads to the problematic accumulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-GALT antibody [EPR12555] (ab178406), expandable thumbnail

    Western blot - Anti-GALT antibody [EPR12555] (ab178406)

    Lanes 1-4: Merged signal (red and green). Green - ab178406 observed at 43 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab178406 Anti-GALT antibody [EPR12555] was shown to specifically react with Galactose-1-Phosphate Uridylyltransferase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265027 (knockout cell lysate ab257958) was used. Wild-type and Galactose-1-Phosphate Uridylyltransferase knockout samples were subjected to SDS-PAGE. ab178406 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: GALT knockout HeLa cell lysate at 20 µg

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: A549 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 43 kDa

    Observed band size: 43 kDa

  • Western blot - Anti-GALT antibody [EPR12555] (ab178406), expandable thumbnail

    Western blot - Anti-GALT antibody [EPR12555] (ab178406)

    Lanes 1 and 2: Green signal from target – ab178406 observed at 43 kDa

    Lanes 3 and 4: Red signal from loading control – Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa

    Lanes 5 and 6: Merged (red and green) signal

    ab178406 was shown to react with GALT when GALT knockout samples were used, along with additional cross-reactive bands. Wild-type and GALT knockout samples were subjected to SDS-PAGE. ab178406 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye® 800CW)preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776)secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    Lanes 1 - 2: Western blot - Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution

    Lanes 3 - 4: Western blot - Anti-GALT antibody [EPR12555] (ab178406) at 1/2000 dilution

    Lanes 1 and 3: GALT knockout HAP1 cell lysate at 20 µg

    Lanes 2 and 4: Wild-type HAP1 cell lysate at 20 µg

    Predicted band size: 43 kDa

  • Immunoprecipitation - Anti-GALT antibody [EPR12555] (ab178406), expandable thumbnail

    Immunoprecipitation - Anti-GALT antibody [EPR12555] (ab178406)

    Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing GALT, immunoprecipitated using ab178406 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-GALT antibody [EPR12555] (ab178406)

    Developed using the ECL technique.

    Predicted band size: 43 kDa

  • Western blot - Anti-GALT antibody [EPR12555] (ab178406), expandable thumbnail

    Western blot - Anti-GALT antibody [EPR12555] (ab178406)

    All lanes: Western blot - Anti-GALT antibody [EPR12555] (ab178406) at 1/1000 dilution

    Lane 1: K562 cell lysate at 10 µg

    Lane 2: MDA-MB-435 cell lysate at 10 µg

    Lane 3: Human fetal brain lysate at 10 µg

    Lane 4: Human fetal liver lysate at 10 µg

    Lane 5: HepG2 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 43 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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