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AB240206

Anti-GALT antibody [EPR12555] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal GALT antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Galactose-1-phosphate uridylyltransferase, Gal-1-P uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase, GALT

2 Images
Immunoprecipitation - Anti-GALT antibody [EPR12555] - BSA and Azide free (AB240206)
  • IP

Supplier Data

Immunoprecipitation - Anti-GALT antibody [EPR12555] - BSA and Azide free (AB240206)

Western blot analysis on Immunoprecipitation pellet from either 1) Human fetal liver lysate, or 2) 1xPBS (negative control); showing GALT, immunoprecipitated using ab178406 at 1/10 dilution with HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178406).

All lanes:

Immunoprecipitation - Anti-GALT antibody [EPR12555] (<a href='/en-us/products/primary-antibodies/galt-antibody-epr12555-ab178406'>ab178406</a>)

Predicted band size: 43 kDa

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Western blot - Anti-GALT antibody [EPR12555] - BSA and Azide free (AB240206)
  • WB

Lab

Western blot - Anti-GALT antibody [EPR12555] - BSA and Azide free (AB240206)

This data was developed using the same antibody clone in a different buffer formulation (ab178406).

Lanes 1-4 : Merged signal (red and green). Green - ab178406 observed at 43 kDa. Red - loading control ab7291 observed at 50 kDa.

ab178406 Anti-GALT antibody [EPR12555] was shown to specifically react with Galactose-1-Phosphate Uridylyltransferase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265027 (knockout cell lysate ab257958) was used. Wild-type and Galactose-1-Phosphate Uridylyltransferase knockout samples were subjected to SDS-PAGE. ab178406 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-GALT antibody [EPR12555] (<a href='/en-us/products/primary-antibodies/galt-antibody-epr12555-ab178406'>ab178406</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

GALT knockout HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12555

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab240206 is the carrier-free version of ab178406.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALT also known as galactose-1-phosphate uridyltransferase is an enzyme involved in the Leloir pathway of galactose metabolism. The GALT protein facilitates the conversion of galactose-1-phosphate (Gal-1-P) and UDP-glucose to UDP-galactose and glucose-1-phosphate important steps in the cellular utilization of galactose. This enzyme has a molecular mass of approximately 43 kDa. GALT is expressed in various tissues and is notably present in the liver and erythrocytes where efficient galactose metabolism is essential.
Biological function summary

GALT plays a role in carbohydrate metabolism by regulating galactose conversion to glucose a principal energy source. It does not form part of a complex but works alongside other enzymes such as galactokinase and UDP-galactose 4'-epimerase in the Leloir pathway. This enzymatic action ensures that galactose from food sources is metabolized properly contributing to overall energy homeostasis within the body.

Pathways

GALT participates in the Leloir pathway which is essential for the breakdown and utilization of galactose. Through this pathway GALT interacts indirectly with related proteins like galactokinase and UDP-galactose 4'-epimerase. This enzymatic pathway is linked to complex carbohydrate metabolism and feeds into broader glucose metabolism processes underlining its importance in maintaining glucose levels.

GALT is linked to classic galactosemia a rare genetic metabolic disorder. This condition arises from an impairment in the GALT enzyme leading to toxic accumulation of galactose-1-phosphate in the body. Individuals with classic galactosemia often experience symptoms like jaundice liver enlargement and can develop cataracts or mental developmental issues if not treated early. In the context of this disorder proteins such as galactokinase also play a role since they are upstream in the metabolic pathway that leads to the problematic accumulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in galactose metabolism.
See full target information GALT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 12:6767-6777 PubMed32801905

2020

Estrogen-Induced Stromal FGF18 Promotes Proliferation and Invasion of Endometrial Carcinoma Cells Through ERK and Akt Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Wu,Xiang Tao,Hong Zhang,Xiang-Hua Yi,Yin-Hua Yu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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