Mouse Polyclonal gamma Adducin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADD3 aa 450-600.
Images
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ADD3 aa 450-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UEY8
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2500 | Notes - |
Species Mouse | Dilution info 1/500 - 1/2500 | Notes - |
Species Rat | Dilution info 1/500 - 1/2500 | Notes - |
Target data
Function
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping (PubMed:23836506). Binds to calmodulin (Probable). Involved in myogenic reactivity of the renal afferent arteriole (Af-art), renal interlobular arteries and middle cerebral artery (MCA) to increased perfusion pressure. Involved in regulation of potassium channels in the vascular smooth muscle cells (VSMCs) of the Af-art and MCA ex vivo. Involved in regulation of glomerular capillary pressure, glomerular filtration rate (GFR) and glomerular nephrin expression in response to hypertension. Involved in renal blood flow (RBF) autoregulation. Plays a role in podocyte structure and function. Regulates globular monomer actin (G-actin) and filamentous polymer actin (F-actin) ratios in the primary podocytes affecting actin cytoskeleton organization. Regulates expression of synaptopodin, RhoA, Rac1 and CDC42 in the renal cortex and the primary podocytes. Regulates expression of nephrin in the glomeruli and in the primary podocytes, expression of nephrin and podocinin in the renal cortex, and expression of focal adhesion proteins integrin alpha-3 and integrin beta-1 in the glomeruli. Involved in cell migration and cell adhesion of podocytes, and in podocyte foot process effacement. Regulates expression of profibrotics markers MMP2, MMP9, TGF beta-1, tubular tight junction protein E-cadherin, and mesenchymal markers vimentin and alpha-SMA (By similarity). Promotes the growth of neurites (By similarity).
Alternative names
ADDL, ADD3, Gamma-adducin, Adducin-like protein 70
Recommended products
Mouse Polyclonal gamma Adducin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human ADD3 aa 450-600.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ADD3 aa 450-600. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UEY8
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma Adducin also known as ADD3 is a protein that plays an important role in the assembly of the spectrin-actin cytoskeleton at the cell membrane. It has a molecular mass of approximately 80 kDa. Gamma Adducin is expressed mainly in brain and kidney tissues but can also be found in other organs in smaller amounts. As a member of the adducin family it shares structural similarities with alpha and beta subunits contributing to diverse cellular processes through its interaction capacities.
- Biological function summary
Gamma Adducin acts as a regulator of the spectrin-actin network and is involved in the stabilization of membrane structures. It participates in the formation of a larger complex with alpha and beta adducins supporting cellular shape and motility. This protein plays roles in cellular processes such as signal transduction cell adhesion and junction integrity. It is also known to affect cellular activities by modifying membrane-cytoskeleton interactions making it an important player in maintaining cell structure.
- Pathways
Gamma Adducin interacts within the Rho signaling pathway and the actin cytoskeleton regulatory pathway. These pathways are essential for cell shape regulation and motility. It works closely with proteins such as actin and spectrin to facilitate cytoskeletal rearrangement and cell signaling networks. Interactions with small GTPases further connect gamma Adducin to pathways that influence changes in cellular architecture.
- Associated diseases and disorders
Gamma Adducin has been linked to hypertension and cardiovascular disease where its function in the membrane skeleton impacts vascular smooth muscle cells' contractility. It interacts with other proteins such as ankyrin and possibly other subunits of adducins in these contexts. Alterations in gamma Adducin expression or mutations can lead to imbalances in cytoskeletal dynamics contributing to disease progression and providing potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-gamma Adducin antibody (ab194426)
All lanes: Western blot - Anti-gamma Adducin antibody (ab194426) at 1/1000 dilution
All lanes: recombinant Adducin protein fragment at 0.2 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Western blot - Anti-gamma Adducin antibody (ab194426)
All lanes: Western blot - Anti-gamma Adducin antibody (ab194426) at 1/500 dilution
All lanes: rat PC12 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Western blot - Anti-gamma Adducin antibody (ab194426)
All lanes: Western blot - Anti-gamma Adducin antibody (ab194426) at 1/500 dilution
All lanes: mouse Raw 264.7 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Western blot - Anti-gamma Adducin antibody (ab194426)
All lanes: Western blot - Anti-gamma Adducin antibody (ab194426) at 1/500 dilution
All lanes: mouse NIH 3T3 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
Western blot - Anti-gamma Adducin antibody (ab194426)
All lanes: Western blot - Anti-gamma Adducin antibody (ab194426) at 1/500 dilution
All lanes: K562 cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 79 kDa
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