AB246992

Anti-gamma Adducin antibody

Name: Anti-gamma Adducin antibody (ab246992) | Abcam
Brand: Abcam Limited
SKU: AB246992
Price: 660 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal gamma Adducin antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ADD3 aa 550-700.

View Alternative Names

ADDL, ADD3, Gamma-adducin, Adducin-like protein 70

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-gamma Adducin antibody (AB246992)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Adducin antibody (AB246992)

PFA-fixed, Triton X-100 permeabilized HEK-293 (human epithelial cell line from embryonic kidney) cells stained for Adducin (green) using ab246992 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labelling gamma Adducin with ab246992 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling gamma Adducin with ab246992 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling gamma Adducin with ab246992 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling gamma Adducin with ab246992 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-gamma Adducin antibody (AB246992)

All lanes:

Western blot - Anti-gamma Adducin antibody (ab246992) at 0.4 µg/mL

All lanes:

RT4 (human urinary bladder cancer cell line) whole cell lysate

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ADD3 aa 550-700. The exact immunogen used to generate this antibody is proprietary information.

Q9UEY8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma Adducin also known as ADD3 is a protein that plays an important role in the assembly of the spectrin-actin cytoskeleton at the cell membrane. It has a molecular mass of approximately 80 kDa. Gamma Adducin is expressed mainly in brain and kidney tissues but can also be found in other organs in smaller amounts. As a member of the adducin family it shares structural similarities with alpha and beta subunits contributing to diverse cellular processes through its interaction capacities.

Biological function summary

Gamma Adducin acts as a regulator of the spectrin-actin network and is involved in the stabilization of membrane structures. It participates in the formation of a larger complex with alpha and beta adducins supporting cellular shape and motility. This protein plays roles in cellular processes such as signal transduction cell adhesion and junction integrity. It is also known to affect cellular activities by modifying membrane-cytoskeleton interactions making it an important player in maintaining cell structure.

Pathways

Gamma Adducin interacts within the Rho signaling pathway and the actin cytoskeleton regulatory pathway. These pathways are essential for cell shape regulation and motility. It works closely with proteins such as actin and spectrin to facilitate cytoskeletal rearrangement and cell signaling networks. Interactions with small GTPases further connect gamma Adducin to pathways that influence changes in cellular architecture.

Gamma Adducin has been linked to hypertension and cardiovascular disease where its function in the membrane skeleton impacts vascular smooth muscle cells' contractility. It interacts with other proteins such as ankyrin and possibly other subunits of adducins in these contexts. Alterations in gamma Adducin expression or mutations can lead to imbalances in cytoskeletal dynamics contributing to disease progression and providing potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping (PubMed : 23836506). Binds to calmodulin (Probable). Involved in myogenic reactivity of the renal afferent arteriole (Af-art), renal interlobular arteries and middle cerebral artery (MCA) to increased perfusion pressure. Involved in regulation of potassium channels in the vascular smooth muscle cells (VSMCs) of the Af-art and MCA ex vivo. Involved in regulation of glomerular capillary pressure, glomerular filtration rate (GFR) and glomerular nephrin expression in response to hypertension. Involved in renal blood flow (RBF) autoregulation. Plays a role in podocyte structure and function. Regulates globular monomer actin (G-actin) and filamentous polymer actin (F-actin) ratios in the primary podocytes affecting actin cytoskeleton organization. Regulates expression of synaptopodin, RhoA, Rac1 and CDC42 in the renal cortex and the primary podocytes. Regulates expression of nephrin in the glomeruli and in the primary podocytes, expression of nephrin and podocinin in the renal cortex, and expression of focal adhesion proteins integrin alpha-3 and integrin beta-1 in the glomeruli. Involved in cell migration and cell adhesion of podocytes, and in podocyte foot process effacement. Regulates expression of profibrotics markers MMP2, MMP9, TGF beta-1, tubular tight junction protein E-cadherin, and mesenchymal markers vimentin and alpha-SMA (By similarity). Promotes the growth of neurites (By similarity).

See full target information ADD3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FEBS open bio 12:1857-1874 PubMed36052535

2022

A cohesin-associated gene score may predict immune checkpoint blockade in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Zhen Liu,Jian-Di Li,Gang Chen,Rong-Quan He,Rui Lin,Zhi-Guang Huang,Jian-Jun Li,Xiu-Fang Du,Xiao-Ping Lv

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-gamma Adducin antibody

Immunocytochemistry/ Immunofluorescence - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Adducin antibody (AB246992)

Western blot - Anti-gamma Adducin antibody (AB246992)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise