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Mouse Recombinant Monoclonal gamma Catenin antibody. Suitable for WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241), expandable thumbnail
  • Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241), expandable thumbnail
  • Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIP
Human
Tested
Tested
Tested
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).

Alternative names

CTNNG, DP3, JUP, Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3

Recommended products

Mouse Recombinant Monoclonal gamma Catenin antibody. Suitable for WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
15/γ-Catenin
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma Catenin also known as Junction Plakoglobin (JUP) is a protein with a molecular mass of approximately 82 kDa. It plays an important role in cell adhesion mechanisms. This protein is expressed widely in various tissue types prominently in epithelial tissues. Gamma Catenin contributes significantly to the structure of adherens junctions and desmosomes physically connecting cells to one another and stabilizing those connections within tissues.

Biological function summary

Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.

Pathways

Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.

Associated diseases and disorders

Alterations in gamma Catenin expression or function relate to conditions such as heart diseases specifically arrhythmogenic right ventricular cardiomyopathy and cancer particularly affecting epithelial tissues. In these conditions abnormal gamma Catenin interactions with other junction proteins like desmoplakin and plakophilin contribute to disease progression. These disruptions suggest its potential as a therapeutic target providing insight into disease mechanisms and opportunities for intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241), expandable thumbnail

    Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:22858986;PMID: 9614196;PMID: 9894611).

    Exposure time:

    Lanes 1/8: 81 seconds
    Lane 2: 26 seconds
    Lanes 3-4: 70 seconds
    Lane 5/7: 15 seconds
    Lane 6: 3 seconds

    All lanes: Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse heart tissue lysate at 10 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Lane 5: Rat brain tissue lysate at 10 µg

    Lane 6: Rat heart tissue lysate at 10 µg

    Lane 7: Human heart tissue lysate at 10 µg

    Lane 8: Human kidney tissue lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 82 kDa

  • Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241), expandable thumbnail

    Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: (PMID: 22858986)

    The expression profile observed is consistent with what has been described in the literature (PMID:22858986; PMID:9614196; PMID:9894611).

    Exposure time:

    Lanes 1-2/5-7: 15 seconds
    Lanes 3-4: 59 seconds
    Lane 8: 3 seconds

    All lanes: Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

    Lane 4: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 10 µg

    Lane 5: A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 6: Calu-1 (human lung epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 7: C2C12 (mouse myoblasts), whole cell lysate at 10 µg

    Lane 8: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 82 kDa

  • Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241), expandable thumbnail

    Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241)

    gamma Catenin was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab305241 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305241 at dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 whole cell lysate 10 µg

    Lane 2: ab305241 IP in MCF7 whole cell lysate

    Lane 3: Mouse IgG2a monoclonal isotype control (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab305241 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 67 seconds.

    All lanes: Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241) at 1/30 dilution

    All lanes: MCF7 (human breast adenocarcinoma epithelial cell)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 82 kDa

    Exposure time: 67s

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling gamma Catenin with ab305241 at 1/100 dilution (9.85 µg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2µg/ml) (Green).

    Confocal image showing cell-junction staining in MCF7 cell line.

    Low expression: THP-1 (PMID: 22858986).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

    -ve control 1: ab305241 at 1/100 dilution followed by at Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 a 1/500 dilution.
    -ve control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at 1/200 dilution followed by at Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 a 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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