Anti-gamma Catenin antibody [15/γ-Catenin]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling gamma Catenin with ab305241 at 1/100 dilution (9.85 µg/ml), followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2µg/ml) (Green). Confocal image showing cell-junction staining in MCF7 cell line. Low expression : THP-1 (PMID : 22858986). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml) followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab305241 at 1/100 dilution followed by at ab150080 a 1/500 dilution. -ve control 2 : ab179513 at 1/200 dilution followed by at ab150117 a 1/1000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241)

gamma Catenin was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab305241 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305241 at dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241) at 1/1000 dilution

Lane 1:

MCF7 whole cell lysate at 10 µg

Lane 2:

ab305241 at 1/30 IP in MCF7 whole cell lysate at 10 µg

Lane 3:

Mouse IgG2a monoclonal isotype control (<a href='/en-us/products/primary-antibodies/mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-ab18413'>ab18413</a>) instead of ab305241 in MCF7 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 82 kDa

false

Exposure time: 67s

• WB

Supplier Data

Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : (PMID : 22858986) The expression profile observed is consistent with what has been described in the literature (PMID : 22858986; PMID : 9614196; PMID : 9894611). Exposure time : Lanes 1-2/5-7 : 15 seconds Lanes 3-4 : 59 seconds Lane 8 : 3 seconds

All lanes:

Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 4:

THP-1 (human monocytic leukemia monocyte), whole cell lysate at 10 µg

Lane 5:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 6:

Calu-1 (human lung epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 7:

C2C12 (mouse myoblasts), whole cell lysate at 10 µg

Lane 8:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 82 kDa

false

• WB

Supplier Data

Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (AB305241)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The expression profile observed is consistent with what has been described in the literature (PMID : 22858986;PMID : 9614196;PMID : 9894611). Exposure time : Lanes 1/8 : 81 seconds Lane 2 : 26 seconds Lanes 3-4 : 70 seconds Lane 5/7 : 15 seconds Lane 6 : 3 seconds

All lanes:

Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (ab305241) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Human heart tissue lysate at 10 µg

Lane 8:

Human kidney tissue lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 82 kDa

false