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Mouse Recombinant Monoclonal gamma Catenin antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (AB305242), expandable thumbnail
  • Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (AB305242), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (AB305242), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIP
Human
Tested
Tested
Tested
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).

Alternative names

CTNNG, DP3, JUP, Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3

Recommended products

Mouse Recombinant Monoclonal gamma Catenin antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
15/γ-Catenin
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab305242 is the carrier-free version of Anti-gamma Catenin antibody [15/γ-Catenin] ab305241.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma Catenin also known as Junction Plakoglobin (JUP) is a protein with a molecular mass of approximately 82 kDa. It plays an important role in cell adhesion mechanisms. This protein is expressed widely in various tissue types prominently in epithelial tissues. Gamma Catenin contributes significantly to the structure of adherens junctions and desmosomes physically connecting cells to one another and stabilizing those connections within tissues.

Biological function summary

Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.

Pathways

Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.

Associated diseases and disorders

Alterations in gamma Catenin expression or function relate to conditions such as heart diseases specifically arrhythmogenic right ventricular cardiomyopathy and cancer particularly affecting epithelial tissues. In these conditions abnormal gamma Catenin interactions with other junction proteins like desmoplakin and plakophilin contribute to disease progression. These disruptions suggest its potential as a therapeutic target providing insight into disease mechanisms and opportunities for intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (ab305242), expandable thumbnail

    Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (ab305242)

    This data was developed using Anti-gamma Catenin antibody [15/γ-Catenin] ab305241, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID:22858986;PMID: 9614196;PMID: 9894611).

    Exposure time:

    Lanes 1/8: 81 seconds
    Lane 2: 26 seconds
    Lanes 3-4: 70 seconds
    Lane 5/7: 15 seconds
    Lane 6: 3 seconds

    All lanes: Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (Anti-gamma Catenin antibody [15/γ-Catenin] ab305241) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 10 µg

    Lane 2: Mouse heart tissue lysate at 10 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

    Lane 5: Rat brain tissue lysate at 10 µg

    Lane 6: Rat heart tissue lysate at 10 µg

    Lane 7: Human heart tissue lysate at 10 µg

    Lane 8: Human kidney tissue lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 82 kDa

  • Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (ab305242), expandable thumbnail

    Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (ab305242)

    This data was developed using Anti-gamma Catenin antibody [15/γ-Catenin] ab305241, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: (PMID: 22858986)

    The expression profile observed is consistent with what has been described in the literature (PMID:22858986; PMID:9614196; PMID:9894611).

    Exposure time:

    Lanes 1-2/5-7: 15 seconds
    Lanes 3-4: 59 seconds
    Lane 8: 3 seconds

    All lanes: Western blot - Anti-gamma Catenin antibody [15/γ-Catenin] (Anti-gamma Catenin antibody [15/γ-Catenin] ab305241) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

    Lane 4: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 10 µg

    Lane 5: A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 6: Calu-1 (human lung epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 7: C2C12 (mouse myoblasts), whole cell lysate at 10 µg

    Lane 8: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 82 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (ab305242), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [15/γ-Catenin] - BSA and Azide Free (ab305242)

    This data was developed using Anti-gamma Catenin antibody [15/γ-Catenin] ab305241, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labeling gamma Catenin with Anti-gamma Catenin antibody [15/γ-Catenin] ab305241 at 1/100 dilution (9.85 µg/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2µg/ml) (Green).

    Confocal image showing cell-junction staining in MCF7 cell line.

    Low expression: THP-1 (PMID: 22858986).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (10µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (4µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-gamma Catenin antibody [15/γ-Catenin] ab305241 at 1/100 dilution followed by at Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 a 1/500 dilution.
    -ve control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 at 1/200 dilution followed by at Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 a 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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