Rabbit Polyclonal gamma Catenin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications.
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Publications
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- Journal of advanced research 28:195-2082020Effects of differential distributed-JUP on the malignancy of gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanlin Chen,Liping Yang,Yilu Qin,Shuiqing Liu,Yina Qiao,Xueying Wan,Huan Zeng,Xiaoli Tang,Manran Liu,Yixuan HouPubMed 33364056
- JCI insight 5:2019Patient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRonald Ng et. al.PubMed 31194698
- Human molecular genetics 25:348-572015Dominant de novo DSP mutations cause erythrokeratodermia-cardiomyopathy syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLynn M Boyden,Chen Y Kam,Angela Hernández-Martín,Jing Zhou,Brittany G Craiglow,Robert Sidbury,Erin F Mathes,Sheilagh M Maguiness,Debra A Crumrine,Mary L Williams,Ronghua Hu,Richard P Lifton,Peter M Elias,Kathleen J Green,Keith A ChoatePubMed 26604139
- PloS one 9:e1154962014Implications of targeted genomic disruption of β-catenin in BxPC-3 pancreatic adenocarcinoma cells.Applications:IPReactive species:Unspecified reactive speciesPetter Angell Olsen,Nina Therese Solberg,Kaja Lund,Tore Vehus,Monika Gelazauskaite,Steven Ray Wilson,Stefan KraussPubMed 25536063
- Science signaling 7:rs72014E-cadherin interactome complexity and robustness resolved by quantitative proteomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenhuan Guo et. al.PubMed 25468996
- The American journal of surgical pathology 34:1472-92010Clinical and biological significance of E-cadherin protein expression in invasive lobular carcinoma of the breast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmad A Rakha,Arjun Patel,Des G Powe,Ahmed Benhasouna,Andrew R Green,Maryou B Lambros,Jorge S Reis-Filho,Ian O EllisPubMed 20871222
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
Alternative names
CTNNG, DP3, JUP, Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3
Recommended products
Rabbit Polyclonal gamma Catenin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma Catenin also known as Junction Plakoglobin (JUP) is a protein with a molecular mass of approximately 82 kDa. It plays an important role in cell adhesion mechanisms. This protein is expressed widely in various tissue types prominently in epithelial tissues. Gamma Catenin contributes significantly to the structure of adherens junctions and desmosomes physically connecting cells to one another and stabilizing those connections within tissues.
- Biological function summary
Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.
- Pathways
Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.
- Associated diseases and disorders
Alterations in gamma Catenin expression or function relate to conditions such as heart diseases specifically arrhythmogenic right ventricular cardiomyopathy and cancer particularly affecting epithelial tissues. In these conditions abnormal gamma Catenin interactions with other junction proteins like desmoplakin and plakophilin contribute to disease progression. These disruptions suggest its potential as a therapeutic target providing insight into disease mechanisms and opportunities for intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody (ab15153)
Human breast carcinoma stained with anti gamma catenin antibody ab15153 (1/100 for 10 min at RT). Gamma catenin staining is seen on the cell membrane and in the cytoplasm.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody (ab15153)
ab15153 staining gamma Catenin in normal human skin tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% serum for 1 hour at 2°C; antigen retrival was by heat mediation in EDTA (pH9). The sample was incubated with primary antibody (1/100 in TBS + 1% BSA) at 21°C for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody (ab15153)
ICC/IF image of ab15153 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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