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Rabbit Recombinant Monoclonal gamma Catenin antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Western blot - Anti-gamma Catenin antibody [EPR17310-48] (AB200661), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] (AB200661), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310-48] (AB200661), expandable thumbnail
  • Western blot - Anti-gamma Catenin antibody [EPR17310-48] (AB200661), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] (AB200661), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/400
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/40
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).

Alternative names

CTNNG, DP3, JUP, Junction plakoglobin, Catenin gamma, Desmoplakin III, Desmoplakin-3

Recommended products

Rabbit Recombinant Monoclonal gamma Catenin antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR17310-48
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma Catenin also known as Junction Plakoglobin (JUP) is a protein with a molecular mass of approximately 82 kDa. It plays an important role in cell adhesion mechanisms. This protein is expressed widely in various tissue types prominently in epithelial tissues. Gamma Catenin contributes significantly to the structure of adherens junctions and desmosomes physically connecting cells to one another and stabilizing those connections within tissues.

Biological function summary

Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.

Pathways

Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.

Associated diseases and disorders

Alterations in gamma Catenin expression or function relate to conditions such as heart diseases specifically arrhythmogenic right ventricular cardiomyopathy and cancer particularly affecting epithelial tissues. In these conditions abnormal gamma Catenin interactions with other junction proteins like desmoplakin and plakophilin contribute to disease progression. These disruptions suggest its potential as a therapeutic target providing insight into disease mechanisms and opportunities for intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661), expandable thumbnail

    Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/20000 dilution

    Lane 1: Human fetal heart lysates at 10 µg

    Lane 2: Human fetal kidney lysates at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa

    Observed band size: 82 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] (ab200661), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] (ab200661)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling gamma Catenin with ab200661 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on Human kidney tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310-48] (ab200661), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310-48] (ab200661)

    Immunocytochemistry analysis of Hela (Human cervix adenocarcinoma epithelial cell) labeling gamma Catenin with purified ab200661 at 1/400 dilution. Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.

  • Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661), expandable thumbnail

    Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/5000 dilution

    All lanes: Human fetal skin lysates at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 82 kDa

    Observed band size: 82 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] (ab200661), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Catenin antibody [EPR17310-48] (ab200661)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling gamma Catenin with ab200661 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on Human cervix carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661), expandable thumbnail

    Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-gamma Catenin antibody [EPR17310-48] (ab200661) at 1/5000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

    Lane 2: T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 82 kDa

    Observed band size: 82 kDa

    Exposure time: 5s

  • Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310-48] (ab200661), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-gamma Catenin antibody [EPR17310-48] (ab200661)

    Intracellular Flow Cytometry analysis of HeLa cells labelling gamma Catenin (red) with purified ab200661at dilution of 1/40. The secondary antibody used was Alexa Fluorr® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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